WEKO3
アイテム
{"_buckets": {"deposit": "81cdb147-bf71-412f-b895-72631d5c04b2"}, "_deposit": {"created_by": 3, "id": "75", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "75"}, "status": "published"}, "_oai": {"id": "oai:kwmed.repo.nii.ac.jp:00000075", "sets": ["332"]}, "author_link": ["127411", "127407", "127413", "127408", "127406", "127409", "127412", "127410", "127405"], "item_3_biblio_info_12": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1983", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "66", "bibliographicPageStart": "61", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{}, {"bibliographic_title": "Kawasaki medical journal", "bibliographic_titleLang": "en"}]}]}, "item_3_description_15": {"attribute_name": "表示順", "attribute_value_mlt": [{"subitem_description": "3", "subitem_description_type": "Other"}]}, "item_3_description_16": {"attribute_name": "アクセション番号", "attribute_value_mlt": [{"subitem_description": "KJ00010227419", "subitem_description_type": "Other"}]}, "item_3_description_9": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_description": "Original Article", "subitem_description_type": "Other"}]}, "item_3_identifier_14": {"attribute_name": "URL", "attribute_value_mlt": [{"subitem_identifier_type": "URI", "subitem_identifier_uri": "http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1983en/9(2)61-66.1983.pdf | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1983en/9(2)61-66.1983.pdf"}]}, "item_3_relation_20": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.11482/KMJ-E9(2)61 ", "subitem_relation_type_select": "DOI"}}]}, "item_3_source_id_1": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00710169", "subitem_source_identifier_type": "NCID"}, {"subitem_source_identifier": "AA12029005", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_19": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0385-0234", "subitem_source_identifier_type": "ISSN"}, {"subitem_source_identifier": "2434-3404", "subitem_source_identifier_type": "ISSN"}]}, "item_3_text_3": {"attribute_name": "論文名よみ", "attribute_value_mlt": [{"subitem_text_value": "Analysis of Human Insulin Analogues in Vitro, Using Gel Chromatographic Method"}]}, "item_3_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Endocrinology, Department of Medicine, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Surgery, Kawasaki Medical School"}]}, "item_3_textarea_11": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_textarea_language": "en", "subitem_textarea_value": "The incubation medium and incubated human pancreas were gel chromatographed on the Bio-Gel P-30 column after extraction with acid ethanol. The extracted immunoreactive insulin (IRI) was successfully separated into two peaks at the position of 6000 molecular weight region. These two peaks corresponded to those which were detected in human serum. These findings suggest that the two groups of insulin are directly secreted from human pancreatic tissue. But the incorporated [3H] leucine peak into acid-ethanol extractable protein did not elute out at the same position as each insulin peak. Therefore, the measurement of [3H] leucine incorporation into acid-ethanol extractable protein is not a good indicator to evaluate insulin biosynthesis. "}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KAKITA, Keiji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127405", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HORINO, Masaharu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127406", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TENKU, Atsuko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127407", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OYAMA, Hideki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127408", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ENDOH, Masahiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127409", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MATSUKI, Michihiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127410", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NAGASE, Yumiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127411", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NISHIDA, Seikoh", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127412", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SANO, Kaiso", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127413", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-01-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00010227419.pdf", "filesize": [{"value": "548.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 548000.0, "url": {"label": "KJ00010227419.pdf", "url": "https://kwmed.repo.nii.ac.jp/record/75/files/KJ00010227419.pdf"}, "version_id": "c62890d3-1711-4c39-88fe-52cf7f3702b3"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "human insulin snalogues", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gel chromatography", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Analysis of Human Insulin Analogues in Vitro, Using Gel Chromatographic Method", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Analysis of Human Insulin Analogues in Vitro, Using Gel Chromatographic Method", "subitem_title_language": "en"}]}, "item_type_id": "3", "owner": "3", "path": ["332"], "permalink_uri": "https://kwmed.repo.nii.ac.jp/records/75", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-01-23"}, "publish_date": "2017-01-23", "publish_status": "0", "recid": "75", "relation": {}, "relation_version_is_last": true, "title": ["Analysis of Human Insulin Analogues in Vitro, Using Gel Chromatographic Method"], "weko_shared_id": -1}
Analysis of Human Insulin Analogues in Vitro, Using Gel Chromatographic Method
https://kwmed.repo.nii.ac.jp/records/75
https://kwmed.repo.nii.ac.jp/records/7545a738a2-299a-4494-9eec-94b93a91fff6
名前 / ファイル | ライセンス | アクション |
---|---|---|
KJ00010227419.pdf (548.0 kB)
|
|
Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-01-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Analysis of Human Insulin Analogues in Vitro, Using Gel Chromatographic Method | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
KAKITA, Keiji
× KAKITA, Keiji× HORINO, Masaharu× TENKU, Atsuko× OYAMA, Hideki× ENDOH, Masahiko× MATSUKI, Michihiro× NAGASE, Yumiko× NISHIDA, Seikoh× SANO, Kaiso |
|||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Division of Endocrinology, Department of Medicine, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Surgery, Kawasaki Medical School | ||||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | human insulin snalogues | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | gel chromatography | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Original Article | |||||
抄録(英) | ||||||
en | ||||||
The incubation medium and incubated human pancreas were gel chromatographed on the Bio-Gel P-30 column after extraction with acid ethanol. The extracted immunoreactive insulin (IRI) was successfully separated into two peaks at the position of 6000 molecular weight region. These two peaks corresponded to those which were detected in human serum. These findings suggest that the two groups of insulin are directly secreted from human pancreatic tissue. But the incorporated [3H] leucine peak into acid-ethanol extractable protein did not elute out at the same position as each insulin peak. Therefore, the measurement of [3H] leucine incorporation into acid-ethanol extractable protein is not a good indicator to evaluate insulin biosynthesis. | ||||||
書誌情報 |
en : Kawasaki medical journal 巻 9, 号 2, p. 61-66, 発行日 1983 |
|||||
URL | ||||||
識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1983en/9(2)61-66.1983.pdf | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1983en/9(2)61-66.1983.pdf | |||||
識別子タイプ | URI | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11482/KMJ-E9(2)61 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-0234 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2434-3404 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00710169 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12029005 |