{"created":"2023-06-19T10:27:57.857800+00:00","id":122,"links":{},"metadata":{"_buckets":{"deposit":"ac57f374-3509-4adf-a115-b44cdde75ac4"},"_deposit":{"created_by":3,"id":"122","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"122"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00000122","sets":["1709617079800:37:56:338"]},"author_link":["127311","127310"],"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1984","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"180","bibliographicPageStart":"169","bibliographicVolumeNumber":"10","bibliographic_titles":[{},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original Article","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1984en/10(3)169-180.1984.pdf | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1984en/10(3)169-180.1984.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-E10(3)169 ","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00710169","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12029005","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0234","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"2434-3404","subitem_source_identifier_type":"ISSN"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Pharmacology, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Pharmacology, Kawasaki Medical School"}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"The effects of molybdate on the binding characteristics of androgen receptor from the androgen-dependent mouse tumor (Shionogi carcinoma 115) with 17/β-hydroxy-5α-androstan-3-one were studied by the charcoal adsorption method. The dissociation constant (Kd) was (4.65±0.54) ×1O-11 M in the presence of 10-12 mM sodium molybdate, which was one order of magnitude lower than the value without molybdate ((4.54±0.72)× 10-10 M). Since the half life of the binding activity of unbound receptor was extended from 10 h to 75 h by adding molybdate, the effects of chaotropic reagents on binding were studied in the presence of molybdate. KC1 (0.3 -1.0 M) remarkably increased Kd without affecting the maximum binding capacity or the dissociation rate. KSCN (>0.1 M) had no effect on Kd but increased the dissociation rate, and while at concentrations at or above 0.5 M it completely inhibited the androgen binding to the cytosol receptor. Urea (0.5-2.0 M) increased Kd proportionally to the increase in the dissociation rate. Kd was also increased by 0.2-0.5 M guanidine, but the maximum binding capacity was reduced. "}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NOHNO, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAITO, Taiichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00010225139.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00010225139.pdf","url":"https://kwmed.repo.nii.ac.jp/record/122/files/KJ00010225139.pdf"},"version_id":"6d71b633-1d7a-43a6-8f37-9c7afde10fd8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Androgen receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sodium molybdate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Steroid binding","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Shionogi carcinoma 115","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115","subitem_title_language":"en"}]},"item_type_id":"3","owner":"3","path":["338"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"122","relation_version_is_last":true,"title":["Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T10:56:42.297133+00:00"}