{"created":"2023-06-19T10:29:09.152646+00:00","id":1265,"links":{},"metadata":{"_buckets":{"deposit":"769c1f50-b2a2-4239-989a-90f288903207"},"_deposit":{"created_by":31,"id":"1265","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1265"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001265","sets":["1709617079800:35:262:505"]},"author_link":["114297","114298"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"183","bibliographicPageStart":"175","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2002/KMJ28(3)175-183.2002.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ28(3)175-183.2002.pdf","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_3_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学泌尿器科"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Urology, Kawasaki Medical School"}]},"item_3_textarea_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"ヒト精巣セミノーマ由来の樹立株である高転移株(JKT-HMを用い,長期,少量,間歇的にcis-diamminedichloroplatinum (II) (CDDP)を投与することによってCDDP耐性株(JKT/DDP)の作成を試みた.その後,original細胞であるヒト精巣セミノーマ株(JKT-1),その高転移株であるJKT-HMと成長曲線,CDDP感受性,CDDP細胞内濃度についてin vitroで比較検討した.その結果,JKT/DDPは通常のCDDP無添加の培養においてはJKT-1, JKT-HMと比較して,成長曲線に差は認めなかった.しかし,CDDP添加による培養では,明らかに成長曲線に差を認めた.また,CDDP存在下で細胞形態を観察すると,JKT-1, JKT-HMでは細胞質が分葉化し,クロマチンの凝集を認めたが,JKT/DDPでは紡錘形の細胞が敷石状の増殖を示していた.CDDPに対する感受性について,IC_<50>, Collagen gel drop embedded drug sensitivity test (CD-DST)法で検討した結果,JKT/DDPは他の2つの細胞株と比較して,有意にCDDP耐性を示した.よって,CDDP耐性細胞を樹立したと考えられた.次に,JKT-1, JKT-HMおよび,JKT-DDPにおけるCDDP細胞内濃度,CDDP細胞質内代謝に関連する抗癌剤耐性関連遺伝子(Multidrug resistance 1, MDR1 : Multidrug resistance associated protein, MRP ; Lung resistance-related protein, LRP ; Glutathione-S-transferase π, GST-π)の発現について検討した.CDDP細胞内濃度はJKT/DDPで他の2種類の細胞よりも低下しており,また,MDR1, MRP, LRP, GST-πのmRNAの発現についてReal Time PCRで検討したところ,JKT/DDPは,JKT-1, JKT-HMに比べてMDR1, MRP, GST-π遺伝子が有意に発現の亢進が認められた。よって,JKT/DDPはJKT-1, JKT-HMに比較して,CDDPに対して耐性化を示しているとともに,CDDPに対する耐性化の機序のひとつにCDDPの細胞外への能動的な排出の亢進が関与していることが示唆された."}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Using a highly metastatic human testicular seminoma cell line, JKT-HM, a CDDP-resistant cell line, JKT/DDP, was established by long-term intermittent administration of a low dose of cisdimminedichloroplatnium (II) (CDDP). CDDP-resistance was investigated in vitro by comparison of the growth curves, CDDP-sensitivity, and intracellular CDDP concentrations of the established cell line, JKT/DDP, the original human seminoma cell line, JKT-1, and its highly metastatic derivative, JKT-HM. The growth curve and doubling time of JKT/DDP were not significantly different from those of JKT-1 or JKT-HM in culture without CDDP. However, in the presence of CDDP in culture, there was a significant difference in the growth curve. Morphological observations revealed segmentation of the cytoplasm and aggregation if chromatin in JKT-1 and JKT-HM. In contrast, in JKT/DDP, spindle-shaped cells showed cobblestone growth. In addition, the IC_<50> values of CDDP determined by the collagen gel drop embedded drug sensitivity test (CD-DST) showed JKT/DDP to be significantly more ersistant to CDDP than the other two cell lines. Therefore, a CDDP-resistant cell line was established. Next, we in investigated the expression of the genes (Multidrug resistance 1, MDR1 ; Multidrug resistance associated protein, MRP ; Lung resistance-related protein, LRP ; Glutathione-S-transferase π, GST-π) related to anticancer drug-resistance associated with the intracellular CDDP concentration and cytoplasmic CDDP metabolism in JKT-1, JKT-HM, and JKT-DDP. The intracellular CDDP concentration was lower in JKT/DDP than in the other two cell lines. mRNA expression of MDR1, MRP, LRP, and GST-π was investigated by a real-time PCR. The expression levels of MDR 1, MRP, and GST-π were significantly higher in JKT/DDP than in JKT-1 and JKT-HM. It was suggested that JKT/DDP acquired CDDP-resistance, and that an increased active exudation of cytoplasmic CDDP was involved in the acquisition of this CDDP-resistance."}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 達也","creatorNameLang":"ja"},{"creatorName":"コバヤシ, タツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KOBAYASHI, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00001725524.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00001725524.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1265/files/KJ00001725524.pdf"},"version_id":"7b441d93-a754-4705-a62e-2373b5cab177"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CDDP-resistant cell line","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Active exudaton of cytoplasmic CDDP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MDR1, MRP, LRP, GST-π","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"cis-diamminedichloroplatinum (II)耐性ヒト精巣セミノーマ株(JKT/DDP)の樹立とReal Time PCRによるCDDP代謝に関連する抗癌剤耐性関連遺伝子の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"cis-diamminedichloroplatinum (II)耐性ヒト精巣セミノーマ株(JKT/DDP)の樹立とReal Time PCRによるCDDP代謝に関連する抗癌剤耐性関連遺伝子の検討","subitem_title_language":"ja"},{"subitem_title":"Establishment of cis-diamminedichloroplatinum (II) - resistant Human Testicular Seminoma Cell Link JKT/DDP and Analysis of the Expression of the Genes Related to Anticancer Drug-resistance associated with Cytoplasmic CDDP Metabolism","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["505"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"1265","relation_version_is_last":true,"title":["cis-diamminedichloroplatinum (II)耐性ヒト精巣セミノーマ株(JKT/DDP)の樹立とReal Time PCRによるCDDP代謝に関連する抗癌剤耐性関連遺伝子の検討"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2024-04-18T04:05:15.054338+00:00"}