{"created":"2023-06-19T10:29:11.068691+00:00","id":1297,"links":{},"metadata":{"_buckets":{"deposit":"600df101-9b89-4ae9-bdb7-57250fe40829"},"_deposit":{"created_by":31,"id":"1297","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1297"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001297","sets":["1709617079800:35:262:506"]},"author_link":["114348","114349","114350","114351","114352","114353"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"286","bibliographicPageStart":"279","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2002/KMJ28(4)279-286.2002.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ28(4)279-286.2002.pdf","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_3_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学外科消化器部門"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学外科消化器部門"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学外科消化器部門"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterological Surgery, Department of Surgery, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterological Surgery, Department of Surgery, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterological Surgery, Department of Surgery, Kawasaki Medical School"}]},"item_3_textarea_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"膵胆管合流異常症は,胆道系癌が発生することで前癌状態として認識されている.その発癌機序の一つとしては,膵液の胆管への逆流が関与していると考えられている.そこで我々は,ハムスターに外科的に胆汁・膵液・十二指腸液が確実に胆道内に逆流する処置を加え,いわゆる膵胆管合流異常症モデル(cholecystoduodenostomy with dessection of the extrahepatic bile duct on the distal end of the common duct以下のCDDBモデル)を作成し,胆汁,膵液を含む十二指腸液の逆流が,胆道上皮へ及ぼす影響を検討した.術後,全てのハムスターに基本飼育を与え,水道水を自由摂取させた.そして発癌剤を投与することなく6ヶ月および12ヶ月以上観察した.病理組織学的には,6ヶ月観察の6例全例に胆嚢上皮の過形成を認め,1例では高度の腺管の異形成が認められた.そして胆嚢粘膜の増殖細胞の検出をPCNA LIを用い測定した結果,CDDBモデルは,手術非施行例に比べて高値であった.肝外胆管上皮は炎症細胞の浸潤はあるものの過形成は認められなかった.肝内胆管,膵管上皮には特別な病変を認めなかった.12ヶ月以上観察できた14例の胆嚢では,全例に胆嚢上皮の過形成がみられ,異形成はみられなかった.一方肝外胆管では,上皮の過形成が14例中半数の7例にみられ,1例に肝外胆肝癌の発生を認めた.しかしK-ras遺伝子変化はみられなかった.6ヶ月に比べ12ヶ月の長期観察では肝外胆管にも変化がみられた.この実験結果より,発癌剤を投与しなくとも,CDDBモデルは逆流した膵液と胆汁を含む十二指腸液が長期間にわたり直接粘膜に接触すると,胆嚢上皮と胆管上皮の細胞回転が亢進し,過形成,異形成が発症し,さらには発癌に至ることが示された.すなわち,この実験モデルは,発癌物質を投与することなく胆道の前癌状態から発癌にいたる過程を観察でき,発癌機構を解明できる有用なモデルとなる可能性がある."}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"It is well known that pancreaticobiliary maljunction (PBM) in the pancreatobiliary system promotes development of biliary carcinoma in man. The reflux of pancreatic juice into the biliary tract is considered to be one factor promoting biliary carcinoma. Therefore, we carried out a cholecystoduodenostomy with dissection of the extrahepatic bile duct at the distal end of the common duct (CDDB) in hamsters in such a way that pancreatic juice and duodenal contents would enter the biliary tract. After with CDDB, all the animals were fed a basal diet and provided drinking water ad libitum without the use of a carcinogen until they were sacrificed at 6 months or 12 months. In the six-month group, hyperplasia of the epithelium in the gallbladder was observed in 100%. Severte dysplasia of the epithelium in the gallbladder was noted in one out of six hamsters. The cell kinetics of the gallbladder epithelium were examined using the PCNA-labeling index (PCNA LI). A high PCNA LI in the epithelium of the gallbladder was demonstrated in hamsters that had undergone CDDB but not in those who had not. Although inflammatory cells permeated the epithelium of the extrahepatic bile duct, no hyperplasia was observed. No pathological findings were made in the liver or pancreas. In the 12 month group, hyperplasia of the ipithelium of the gallbladder was observed in 100% and of the extrahepatic bile duct in 50%. Extrahepatic bile duct carcinoma was found in 1 out of 14 hamsters. K-ras gene mutation was not detected. In conclusion, the CDDB procedure itself greatly accelerated cell turn-over of the epithelium in the extrahepatic biliary system. We could observe extrahepatic biliary carcinogenesis in the model without a carcinogen. This model allows for observation of the process from hyperplasia to carcinoma without the use of a carcinogen, and is a useful model for investigating and clarifying the mechanism of carcinogenesis in the extrahepatic biliary sytem."}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹尾, 智行","creatorNameLang":"ja"},{"creatorName":"タケオ, トモユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"真嶋, 敏光","creatorNameLang":"ja"},{"creatorName":"マジマ, トシミツ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"角田, 司","creatorNameLang":"ja"},{"creatorName":"ツノダ, ツカサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAKEO, Tomoyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MAJIMA, Toshimitsu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUNODA, Tsukasa","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00001725556.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00001725556.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1297/files/KJ00001725556.pdf"},"version_id":"f533800d-5f94-4d9d-be72-71598f765008"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Pancreaticobiliary maljunction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CDDB","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Carcinogen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Extrahepatic biliary carcinogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ハムスター胆汁・膵液・十二指腸液胆道内逆流モデルにおける胆道上皮の組織学変化と発癌","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ハムスター胆汁・膵液・十二指腸液胆道内逆流モデルにおける胆道上皮の組織学変化と発癌","subitem_title_language":"ja"},{"subitem_title":"Carcinogenesis and Change in the Biliary Epithelium in a Hamster CDDB Model without Carcinogen","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["506"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"1297","relation_version_is_last":true,"title":["ハムスター胆汁・膵液・十二指腸液胆道内逆流モデルにおける胆道上皮の組織学変化と発癌"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2024-04-19T03:08:03.053446+00:00"}