{"created":"2023-06-19T10:29:31.493053+00:00","id":1631,"links":{},"metadata":{"_buckets":{"deposit":"56b9c109-f6ce-4a8d-9cce-dc20459c6b50"},"_deposit":{"created_by":31,"id":"1631","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1631"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001631","sets":["1709617079800:35:307:555"]},"author_link":["111599","111600","111601"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"31","bibliographicPageStart":"21","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2013/KMJ31(1)21-31.2013.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/2013/KMJ31(1)21-31.2013.pdf","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Public Health, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Public Health, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Molecular and Developmental Biology, Kawasaki Medical School"}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Many epidemiological studies show that genistein intake is effective for maintaining bone mineral density (BMD). Because the reason for the efficacy of genistein as a bone protective agent in vivo remains unclear, we investigated the mechanisms underlying the effects of genistein on BMD in relation to BMP-Smad signaling systems. When osteoblastic MC3T3-E1 cells were exposed to 1 μM genistein, they increased in number. Combined administrations of 1 μM genistein and 1 μM of ICI 182,780 inhibited the increase in cell numbers. Alkaline phosphatase (ALP) and Alizarin red staining showed high activities, indicating that genistein might promote estrogenic differentiation of MC3T3-E1 cells. Moreover, ELISA determined that production of osteoprotegerin (OPG), which is expressed by osteoblasts, was higher when 1 μM genistein was added to the medium than in controls. In contrast, when 10 ng/mL of noggin was administered in the medium, OPG production was inhibited. In order to clarify the underlying mechanism, we investigated the BMP-Smad signaling pathway. When genistein was added to the medium, it induced gene expression of BMP-4. Immunofluorescence staining showed that genistein induced phosphorylation of Smad 1/5, a downstream molecule of BMP. When noggin, which binds to BMP and blocks BMP signaling, was added to the medium, phosphorylation of Smad 1/5 was reduced. These results indicate that genistein may regulate bone metabolism through the BMP-Smad signaling pathway as well as through the estrogen receptor pathway."}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hinenoya, Hajime","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katsuyama, Hironobu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nohno, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00008396999.pdf","filesize":[{"value":"614.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00008396999.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1631/files/KJ00008396999.pdf"},"version_id":"92789778-e652-4b3a-a684-3aa8f95eeace"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Genistein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoprotegerin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BMP-Smad signaling","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MC3T3-E1 cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genistein affects osteoblastic MC3T3-E1 cells both through estrogen receptor and BMP-Smad signaling pathways","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genistein affects osteoblastic MC3T3-E1 cells both through estrogen receptor and BMP-Smad signaling pathways","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["555"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"1631","relation_version_is_last":true,"title":["Genistein affects osteoblastic MC3T3-E1 cells both through estrogen receptor and BMP-Smad signaling pathways"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2023-10-11T03:27:28.064113+00:00"}