{"created":"2023-06-19T10:29:33.061739+00:00","id":1658,"links":{},"metadata":{"_buckets":{"deposit":"7262ded7-ec73-4b4c-8e17-1ea479c8d883"},"_deposit":{"created_by":31,"id":"1658","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1658"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001658","sets":["1709617079800:35:321:560"]},"author_link":["111198","111199","111200","111201","111202","111203","111204","111205"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"75","bibliographicPageStart":"67","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2014/KMJ-J40(2)67.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-J40(2)67","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_3_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学第6学年"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学解剖学"},{"subitem_text_language":"ja","subitem_text_value":"福島県立医科大学医学部附属生体情報伝達研究所生体機能研究部門"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学解剖学"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Anatomy, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Anatomy, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Anatomy, Kawasaki Medical School"}]},"item_3_textarea_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"匂いの情報は嗅細胞軸索を介して,嗅球表面の糸球体に入り投射ニューロンの樹状突起とシナプスし,さらに糸球体を構成する様々な神経化学物質を含有するニューロンによって調整される.臨床の場において,慢性副鼻腔炎や鼻茸等で長期間鼻が遮蔽されると症状改善後に嗅覚異常を認めることがある.また,げっ歯類の片鼻閉実験では,糸球体近傍ニューロンのtyrosine hydroxylase(TH)の発現が低下・消失することが知られている.そこで本研究では,マウス及びTH発現をgreen fluorescent protein(GFP)でモニターした遺伝子改変マウス(TH-GFPマウス)を用い,機能的遮断を施したモデルマウスを作製し,入力遮断によるその他の神経化学物質に対する影響を調べた.方法は,縫合によりマウスの左鼻腔を完全に鼻閉させ,3週間～6ヶ月後に灌流固定し,左右嗅球の連続スライス作製後各種抗体を用いて免疫染色を行った.その結果,これまでの報告と同様3週間の鼻閉モデルマウスでは,鼻閉同側嗅球のTH発現が著しく低下しており,左鼻腔の入力遮断がなされていたことが示された.また6ヶ月と長期の鼻閉モデルマウスでは,鼻閉同側だけではなく対側嗅球でも著しいTH発現低下が確認された.TH-GFPマウスでは,THの発現が低下しているにもかかわらずGFP発現を維持する細胞が少数見られ,野生型鼻閉モデルマウスでも同様にTH発現を維持するニューロンが稀に見られた.このようなTHニューロンは発現低下を示すものよりも比較的大きい細胞体を持つ傾向にあった.以上の結果,THニューロンが示す入力遮断に対する反応の多様性は,THニューロンが入力刺激に対して異なる電気特性を生じるという我々の最近の研究結果を支持するものと考える.今後,多様性を示すTHニューロンが匂い入力調節にどのように関わるか解析を進めていきたい."}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"It is well known that one of chemical marker in juxtaglomerular cells in the rodent olfactory bulb (OB), tyrosine hydroxylase (TH), is decreased in its expression after olfactory input deprivation. In the present study, we have made relatively long term olfactory deprivation models, more than 3 weeks to months, by doing nasal closure on wild type and transgenic mice. In combination with immunocytochemistry for neuronal markers such as glutamic acid decalboxylase, calbindin, and olfactory marker protein, we have confirmed that only TH-expression is decreased after deprivation. In our experiment, however, interesting findings were obtained from deprived transgenic mice which express green fluorescent protein (GFP) under the control of the TH promoter (TH-GFP mice). Although GFP-fluorescence indicating TH gene expression and immune-reactivity for TH were typically decreased, immunereactivity for GFP was maintained. Longer deprivation had variable effects of expression of bulbar neurons not only on the deprived side but also on the non-deprived side. In addition, the neurons that expressed GFP even after deprivation have relatively large somata. These findings indicate possible heterogeneity in TH neurons in reaction to odor deprivation and support our recent report of heterogeneous reaction patterns of TH-neurons to olfactory nerve stimulation. We will continue this work by altering conditions of deprivation to clarify the heterogeneity of bulbar neurons organizing OB neural circuitry."}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"谷口, 美季","creatorNameLang":"ja"},{"creatorName":"タニグチ, ミキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"清蔭, 恵美","creatorNameLang":"ja"},{"creatorName":"キヨカゲ, エミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小林, 和人","creatorNameLang":"ja"},{"creatorName":"コバヤシ, カズト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"樋田, 一徳","creatorNameLang":"ja"},{"creatorName":"トイダ, カズノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taniguchi, Miki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kiyokage, Emi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Kazuto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toida, Kazunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00009576219.pdf","filesize":[{"value":"635.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00009576219.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1658/files/KJ00009576219.pdf"},"version_id":"1a632b3f-8e75-4605-8c38-6f72e2e202cb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"鼻閉","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"嗅球糸球体","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"嗅入力遮断","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"チロシン水酸化酵素","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"グルタミン酸脱炭酸酵素","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Naris closure","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Olfactory glomeruli","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Odor deprivation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tyrosine hydroxylase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glutamic acid decarboxylase","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"嗅覚系脳神経回路の解明 : 鼻閉モデルマウスを用いた嗅入力遮断効果の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"嗅覚系脳神経回路の解明 : 鼻閉モデルマウスを用いた嗅入力遮断効果の解析","subitem_title_language":"ja"},{"subitem_title":"Olfactory Brain Circuitry : Analysis of the odor deprivation effect using the naris closed mouse model","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["560"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"1658","relation_version_is_last":true,"title":["嗅覚系脳神経回路の解明 : 鼻閉モデルマウスを用いた嗅入力遮断効果の解析"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2023-10-11T03:04:48.886702+00:00"}