{"created":"2023-06-19T10:29:33.176354+00:00","id":1660,"links":{},"metadata":{"_buckets":{"deposit":"57b5e400-42eb-40a2-b9f3-c1d0234dc516"},"_deposit":{"created_by":31,"id":"1660","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1660"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001660","sets":["1709617079800:35:321:560"]},"author_link":["111208","111209","111210","111211","111212","111213"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"102","bibliographicPageStart":"89","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2014/KMJ-J40(2)89.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-J40(2)89","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_3_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学解剖学"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学解剖学"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学解剖学"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of anatomy, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of anatomy, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of anatomy, Kawasaki Medical School"}]},"item_3_textarea_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"嗅球は明瞭な層構造を持ち,少数のニューロン種から構成され,そこには豊富な化学物質を含むことがわかっており,脳神経回路の解析に有用な領域である.匂い情報を処理する嗅球は,脳の他領域から複数の遠心性ニューロンによる入力を受けており,この一つがセロトニンを含有するニューロン(セロトニンニューロン)である.セロトニンニューロンは,脳全体に広範囲に分布し様々な脳機能の調節を行っており,嗅球においては非対称性シナプスを形成し,嗅覚情報調節に関わっていると考えられている.しかし,このシナプスについては嗅覚調節機構と共に詳細な解析はなされていない.そこで,本研究ではセロトニンニューロンによるシナプスの微細構造を,免疫電子顕微鏡法と電子線トモグラフィーを用いて解析した.また,非対称性シナプスを示すことから,神経伝達物質としてのグルタミン酸の可能性を検討するため,セロトニンとVGLUT3(vesicular glutamate transporter 3)に対する多重蛍光免疫染色法で解析した.セロトニンニューロンによるシナプスは,多くは球形のシナプス小胞を持つが,扁平なものや有心性小胞も存在した.更に,既知のグルタミン酸作動性ニューロンによる非対称性シナプスと比べて,シナプス後肥厚の厚さの多様性が顕著で,シナプス間隙は狭く,シナプスの直径は小さかった.また,セロトニンニューロンの約半数はVGLUT3免疫陽性であり,神経タンパクを含有する有心性小胞を持っていることから,複数の神経伝達物質を含むことが示唆された.シナプス後肥厚は伝達物質であるグルタミン酸の刺激によって厚くなる.セロトニンニューロンは,グルタミン酸を含む複数の神経伝達物質を持つために,グルタミン酸だけを神経伝達物質として持つニューロンが形成する典型的な非対称性シナプスに比べて,多様性のある非対称性シナプスを形成していると考えられる."}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Because of its simple and distinct layers organized by a few types of neurons and its diverse chemical neuroactive substances, the olfactory bulb (OB) is one of the most desirable regions in which to analyze neuronal organization of the brain. The OB is the primary region that processes odor information and consists of olfactory receptor neurons, projection neurons, interneurons and centrifugal neurons. It is well known that the OB is regulated not only by interneurons but also by centrifugal afferents from other brain regions. Serotonergic fibers derived from the raphe nucleus, one of the centrifugal afferents from other brain regions, the OB is highly innervated by serotonergic fibers. These terminals make asymmetrical synapses onto the target neurons in various synapse formations. However, how different these synapses are from typical asymmetrical and how these differences are related to serotonergic function remains to be clarified. The aim of the present study is to accurately assess the morphometry of the synaptic fine structure of serotonergic neurons as compared with olfactory receptor neurons and projection neurons such as mitral/tufted cells. The synapses were analyzed by pre-embedding immuo-electron microscopy and electron tomography which enables analysis of the synapses in more detail. Additionally, the neurotransmitters of serotonergic neurons were analyzed by immunofluorescence. It was shown that the most common shape of synaptic vesicles of serotonergic fibers was round; the synaptic vesicles of olfactory nerves and dendrites of projection neurons were only round. Synaptic clefts of serotonergic fibers were narrower than that of projection neurons. Postsynaptic density (PSD) of serotonergic synapses was very different from that of olfactory receptor neurons and projection neurons. The diameter of serotonergic synapses, the width of the PSD, was smaller than that of the olfactory receptor neurons. Immunofluorescent study revealed that serotonergic varicosities occasionally co-expressed vesicular glutamate transporter 3 (VGLUT3). This indicates that serotonergic neurons have at least two neurotransmitters, serotonin and glutamate. Dense-core vesicles were characterized as containing monoamines and neuropeptides. In contrast, olfactory receptor neurons and projection neurons, which have been well known to exhibit typical asymmetrical synapses, may have use only one transmitter. PSD is reported to be thickened by stimulation of glutamate. Together with findings of previous studies, the present study suggests that serotonergic synapses might release multiple substances: serotonin, glutamate, and a neuropeptide. Due to these multiple substances, serotonergic neurons may various forms of synapses."}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 良典","creatorNameLang":"ja"},{"creatorName":"スズキ, ヨシノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"清蔭, 恵美","creatorNameLang":"ja"},{"creatorName":"キヨカゲ, エミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"樋田, 一徳","creatorNameLang":"ja"},{"creatorName":"トイダ, カズノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Yoshinori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kiyokage, Emi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toida, Kazunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00009576259.pdf","filesize":[{"value":"778.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00009576259.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1660/files/KJ00009576259.pdf"},"version_id":"3f1c0bb3-d8de-49d1-acd0-2d97e049416a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"セロトニン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"嗅球","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"シナプス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"電子線トモグラフィー","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"神経回路","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Serotonin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Synapse","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Electron Tomography","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Olfactory bulb","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neural circuit","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マウス嗅球神経回路におけるセロトニンニューロンのシナプスの微細構造解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウス嗅球神経回路におけるセロトニンニューロンのシナプスの微細構造解析","subitem_title_language":"ja"},{"subitem_title":"Ultrastructural analysis of serotonergic synapses in the mouse olfactory bulb","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["560"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"1660","relation_version_is_last":true,"title":["マウス嗅球神経回路におけるセロトニンニューロンのシナプスの微細構造解析"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2023-10-11T03:04:55.939434+00:00"}