{"created":"2023-06-19T10:29:44.045771+00:00","id":1830,"links":{},"metadata":{"_buckets":{"deposit":"94ea96c9-8be0-4ce2-84f9-485dff569067"},"_deposit":{"created_by":3,"id":"1830","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1830"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001830","sets":["1709617079800:37:41:45"]},"author_link":["125285","125282","125283","125281","125284","125280","125277","125286","125278","125279"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"45","bibliographicPageStart":"31","bibliographicVolumeNumber":"42","bibliographic_titles":[{},{"bibliographic_title":"Kawasaki Medical Journal","bibliographic_titleLang":"en"}]}]},"item_10001_description_34":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Regular Article","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Kawasaki Medical Society"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-E42(2)31","subitem_relation_type_select":"DOI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12685295","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12029005","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0234","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"2434-3404","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Dermatology, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Molecular Diagnostics, Kitasato University School of Allied Health Sciences"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology 1, Kawasaki Medical School"}]},"item_10001_textarea_5":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Sphingosine-1-phosphate (S1P) is a potent lipid mediator that has been implicated in the migration of lymphocytes and endothelial cells through S1P receptors. S1PR1 is strongly expressed in angiosarcoma, a malignant tumor of endothelial cell origin that has a high propensity for metastasis and poor prognosis; however, the pathological significance of S1PR1 expression is not clear. In this study, we investigated the effect of S1PR1 modulation on cell migration, and examined its potential role as a therapeutic target against metastatic dissemination of angiosarcoma. S1PR1 expression in the human angiosarcoma cell line MO-LAS was assessed by immunocytochemical examination and Western blotting. Effects of S1PR1- specific small interfering RNA (siRNA) and that of FTY720-P (a functional S1PR1-antagonist) on MO-LAS cell chemotactic migration towards sphingosine-1-phosphate (S1P) or 10% fetal bovine serum (FBS) were assessed by Transwell migration assay; wound healing assays for random cell migration were performed using a live cell analyzer. Immunostaining revealed high expression of S1PR1 on the MO-LAS cell membrane. Transwell and wound-healing assays showed that S1P enhanced chemotactic and random migration of MO-LAS cells, respectively. Inhibition of S1PR1 expression with siRNA significantly attenuated chemotaxis of cells towards S1P and 10% FBS. Further, FTY720-P strongly induced the internalization and degradation of S1PR1 even in the presence of serum containing S1P. It attenuated chemotactic cell migration of MO-LAS towards both S1P and serum, as well as the random motility of cells at nanomolar concentrations. These results suggest that the S1P/S1PR1 axis may be a potential therapeutic target for inhibition of angiosarcoma metastasis by controlling its cell motility."}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OKA, Daigo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KORESAWA, Risa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJIWARA, Hideyo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NISHIMURA, Hirotake","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IREI, Isao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"AKIYAMA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HAMAZAKI, Shuji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJIMOTO, Wataru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MASUZAWA, Mikio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SADAHIRA, Yoshito","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-09"}],"displaytype":"detail","filename":"KMJ-E42(2)31.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmed.repo.nii.ac.jp/record/1830/files/KMJ-E42(2)31.pdf"},"version_id":"b25183c0-0e8b-44fa-a410-9fdd4f0ffcee"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Angiosarcoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sphingosine-1-phosphate receptor 1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FTY720-phosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Migration","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Metastasis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sphingosine-1-phosphate receptor 1 expression in angiosarcoma : Possible role in metastasis and a potential therapeutic target","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sphingosine-1-phosphate receptor 1 expression in angiosarcoma : Possible role in metastasis and a potential therapeutic target","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["45"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-03-09"},"publish_date":"2017-03-09","publish_status":"0","recid":"1830","relation_version_is_last":true,"title":["Sphingosine-1-phosphate receptor 1 expression in angiosarcoma : Possible role in metastasis and a potential therapeutic target"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T11:07:51.502178+00:00"}