{"created":"2023-06-19T10:29:44.456132+00:00","id":1838,"links":{},"metadata":{"_buckets":{"deposit":"640b8c21-211d-4746-9942-24cab7ad7941"},"_deposit":{"created_by":31,"id":"1838","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1838"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001838","sets":["1709617079800:35:325:597"]},"author_link":["110743","110744","110745","110746","110747","110748"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"93","bibliographicPageStart":"85","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_10001_description_33":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著論文","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10001_description_34":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Regular Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"川崎医学会","subitem_publisher_language":"ja"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-J42(2)85","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2016/KMJ-J42(2)85.pdf","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_10001_text_31":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学眼科学１"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学眼科学１"},{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学中央研究センター　バイオイメージングユニット"}]},"item_10001_text_32":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Ophthalmology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Ophthalmology 1, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Bio imaging unit, Kawasaki medical school"}]},"item_10001_textarea_5":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"The retinal pigment epithelium (RPE) plays an important role in maintaining photoreceptors, and RPE dysfunction causes eye diseases such as age-related macular degeneration (AMD). RPE transplantation has garnered much attention in recent years as a way to replenish healthy RPE, and clinical applications for RPE transplantation in patients with AMD have been reported. Preclinical studies use animal models to examine treatment efficacy, however, whether conventional methods with animal models are suitable for examining RPE transplantation is controversial. Systemic administration of sodium iodate (NaIO3) has been\nshown to induce RPE degeneration in rodent models, recapitulating morphological atrophy in corresponding areas. However, as rodents lack a macular region, these studies cannot precisely evaluate the effects of systemic administration. The induction of blindness in both eyes in nonhuman primates bearing a macular region is also inappropriate in terms of animal ethics. With this in mind, we investigated whether intravitreal injection of NaIO3 can selectively induce a single-eye impairment in an RPE animal model. Additionally, we investigated whether subretinal transplantation of human induced pluripotent stem cells-derived RPE (hiPS-RPE) can protect against NaIO3-induced RPE degeneration.\nThe intravitreal injection of NaIO3 was performed in 8-10 week-old male Wistar rats with rats being grouped by dose (0.005, 0.01, and 0.02 mg/μl). We measured outer nuclear layer (ONL) thickness and inner nuclear layer (INL) thickness at 8 retinal points by hematoxylin-eosin staining and evaluated RPE morphology by transmission electron microscopy sequentially (3, 7, 14, and 28 days after injection). In addition, hiPSC-RPE cell suspension was injected into the subretinal space 2 days after NaIO3 administration and histological evaluation was performed sequentially (7, 14, and 28 days after NaIO3 injection).\nThe results showed no significant changes in INL and ONL thicknesses in the 0.005 mg/μl group. Retinal thicknesses in the 0.02 mg/μl group had almost completely degenerated. In the 0.01 mg/μl group, ONL thickness had almost completely degenerated, but, in contrast INL thickness was preserved as well as 0.005 mg/μl injection group. RPE morphological changes and TUNEL-positive cells appeared at 3 days after NaIO3 injection, followed by rapid thinning of ONL thickness. None of the retinal thickness of contralateral eye in all groups showed any changes. Additionally, ONL thicknesses in the transplanted eyes were significantly greater than those in the nontransplanted and sham-surgery groups."}]},"item_10001_textarea_6":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"網膜色素上皮（RPE）は視細胞維持に重要な役割を担い，その機能低下は加齢黄斑変性等の原因となる．近年，健常なRPE を補充するRPE 移植が注目されており，臨床応用も開始された．前臨床研究における動物実験にあたり，過去にもげっ歯類のヨウ素酸ナトリウム（NaIO3）誘因RPE 変性モデルの研究はあるが，げっ歯類は黄斑部を欠くため最適でない．黄斑を有する非ヒト霊長類における薬剤全身投与は両眼の失明を招くため動物愛護の観点から不適切であり，現在適切なモデルが存在しない．従って，我々はサルを用いた移植実験を念頭に置きNaIO3の硝子体内投与によりラット片眼にRPE 変性を誘発可能か研究した．また，NaIO3誘因RPE 変性モデルにヒト多能性幹細胞由来RPE（hiPS-RPE）を網膜下移植し視細胞の保護効果を検討した．\n8-10週齢のオスのWistar ラットの片眼に0.005，0.01，0.02 mg/μl のNaIO3を硝子体内投与した．経時的にHE 染色にて網膜外層厚と網膜内層厚を計測し，透過型電子顕微鏡で形態学的評価を行った．NaIO3投与後2日目にhiPS-RPE 懸濁液を網膜下に移植し，組織学的評価を行った．\n0.005 mg/μl 投与群では網膜内層・外層厚ともに変化はなく，0.02 mg/μl 投与群では網膜全層が障害され，0.01 mg/μl 投与群では網膜外層のみが障害された．RPE の形態学的変化はNaIO3投与後3日目から出現し，以降網膜外層厚が急速に菲薄化した．いずれの投与群も非投与眼に影響はなかった．hiPS-RPE 移植眼では，網膜外層厚が厚い傾向にあった．\nまとめると，NaIO3片眼硝子体内投与により，げっ歯類片眼RPE 障害モデル作製に成功した．hiPS-RPE 網膜下移植により視細胞保護効果を得られた．本方法はRPE 移植の前臨床研究の動物モデルの基礎として有用である．"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石田, 順子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"110743","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"桐生, 純一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"110744","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須田, 泰司","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"110745","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ISHIDA, Junko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110746","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KIRYU, Junichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110747","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SUDA, Taiji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110748","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-09"}],"displaytype":"detail","filename":"KMJ-J42(2)85.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1838/files/KMJ-J42(2)85.pdf"},"version_id":"a0fec962-ee2a-4ffa-91a3-f140e7908772"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"網膜色素上皮細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ヨウ素酸ナトリウム","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"加齢黄斑変性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"移植","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒトiPS由来RPE","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Retinal pigment epithelium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sodium iodate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Age-related macular degeneration","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transplantation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HiPSC-derived RPE","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"iPS 細胞由来網膜色素上皮細胞移植のためのNaIO3誘因網膜色素上皮変性げっ歯類モデルの特性評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"iPS 細胞由来網膜色素上皮細胞移植のためのNaIO3誘因網膜色素上皮変性げっ歯類モデルの特性評価","subitem_title_language":"ja"},{"subitem_title":"Characterization of a NaIO3-induced retinal pigment epithelium degeneration rodent model for iPSC-RPE cell transplantation","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["597"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-03-09"},"publish_date":"2017-03-09","publish_status":"0","recid":"1838","relation_version_is_last":true,"title":["iPS 細胞由来網膜色素上皮細胞移植のためのNaIO3誘因網膜色素上皮変性げっ歯類モデルの特性評価"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2024-04-16T02:02:55.184601+00:00"}