{"created":"2023-06-19T10:29:48.492851+00:00","id":1915,"links":{},"metadata":{"_buckets":{"deposit":"afe23b52-4b87-4ea4-a928-af7514997f54"},"_deposit":{"created_by":31,"id":"1915","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1915"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001915","sets":["1709617079800:35:616:617"]},"author_link":["110097","110098"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_10001_description_33":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著論文","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10001_description_34":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Regular Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"川崎医学会","subitem_publisher_language":"ja"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-J44(1)1","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2018/KMJ-J44(1)1.pdf","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_10001_text_31":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学泌尿器科学"}]},"item_10001_text_32":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Urology, Kawasaki Medical School"}]},"item_10001_textarea_5":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"　Indoleamine 2,3-dioxygenase (IDO) catalyzes the first and rate-limiting step of tryptophan catabolism and has been implicated in immune tolerance. IDO is induced in various tissues during systematic bacterial infection and plays a key role in immune response. In a previous study, we showed that epididymal IDO expression in mouse is restricted to the caput region. We hypothesize that IDO plays a central role in local immunological reactions in the epididymis. To examine this hypothesis, we investigated several cytokines in an epididymitis model in an IDO knockout mouse (Ido- /- ).\n　Twelve-week-old male mice were used in the study. Wild type mice (Ido+ /+ ) and Ido- /- were injected with lipopolysaccharide (LPS 100 μg) in the epididymis on the side of the vas deferens. After LPS injection, the epididymis was removed in a time-dependent manner. Expression of IDO in the epididymis was chronologically analyzed using ELISA methods. Inflammatory changes were analyzed using a comprehensive cytokine/chemokine assay to identify representative candidates, and immunohistological changes were then analyzed separately for each of these candidates.\n　Histological analysis showed invasion of lymphocyte-predominant inflammatory cells and destruction of epididymal ductal structure in the epididymitis model in Ido+ /+ compared with Ido- /- . IDO assay showed significant up-regulation of IDO were observed in Ido+ /+ compared with Ido+ /+ sham at day1 and day3. On the other hand, there were not observed the expression of IDO in Ido- /- anytime in time series. The comprehensive cytokine/chemokine assay identified six significant markers: pro-inflammatory cytokines (IL-1α, IL-6) and chemokines (CCL3, CXCL1) were downregulated and anti-inflammatory cytokines (IL-4, IL-10) were up-regulated in Ido- /- compared with Ido+ /+ . Similar results were obtained from immunohistochemical staining for these molecules.\n　IDO is involved in epididymal immunological reactions via cytokines/chemokines, and inhibition of IDO may contribute to protection of epididymis tissue when inflammation occurs in this tissue. Therefore, IDO might be a novel target for therapy for epididymitis, in addition to antimicrobial agents."}]},"item_10001_textarea_6":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"　Indoleamine 2,3-dioxygenase（IDO）は，必須アミノ酸であるトリプトファンのキヌレニン代謝経路における律速酵素であり，免疫反応への関連性が示唆されている．これまでに我々は，IDO が精巣上体頭部に高く発現すると報告している．しかし，その役割について明らかにされていない．今回，我々は精巣上体炎におけるIDO の役割について検討した．\n　実験動物は12週齢，雄性，C57BL/6マウス，野生型マウス（Ido+ /+）およびIDO ノックアウトマウス（Ido- /-）を用いた．精管側から精巣上体へlipopolysaccharide（LPS）を投与することで，精巣上体炎モデルを作成し，LPS 投与後に精巣上体を摘出した．精巣上体におけるIDO の経時的な発現についてIDO 定量検査（ELISA 法）を用いて解析した．また，精巣上体における炎症性変化について，網羅的サイトカイン/ ケモカイン定量検査により代表マーカーを選出し，免疫染色を行うことで，精巣上体炎におけるIDO の働きについて生化学的および免疫組織学的に検討した．\n　HE 染色では，Ido- /- はIdo+ /+ と比較して，リンパ球優位の炎症細胞浸潤および精巣上体内の腺管構造破壊が抑制されていた．IDO 定量検査（ELISA 法）では，Ido+ /+ はIdo+ /+ sham と比較して，LPS 投与後１日目，３日目において，精巣上体内におけるIDO 発現の有意な上昇を認めた．Ido- /- では全ての観察期間においてIDO の発現を認めなかった．網羅的サイトカイン/ ケモカイン定量検査では，Ido- /- においてIdo+ /+ と比較して，炎症促進性サイトカイン（IL-1α，IL-6）およびケモカイン（CCL3，CXCL1）の発現が抑制され，炎症抑制性サイトカイン（IL-4，IL-10）の発現が促進されていた．各種マーカーを用いた免疫染色においても同様に，炎症促進性サイトカイン（IL-1α，IL-6）およびケモカイン（CCL3，CXCL1）の発現が抑制され，炎症抑制性サイトカイン（IL-4，IL-10）の発現が促進されていた．\n　精巣上体では，IDO を介したサイトカイン/ケモカインの調節が行われている可能性がある．IDO を阻害することにより，精巣上体炎に対して精巣上体内の組織保護作用を有すると推測され，抗菌薬に加えてIDO 阻害薬が新規治療薬となる可能性があると考えられる． "}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大平, 伸","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHIRA, Shin","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-14"}],"displaytype":"detail","filename":"KMJ-J44(1)1.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1915/files/KMJ-J44(1)1.pdf"},"version_id":"aaf843ac-fc28-4704-8fde-9980714fc6c9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精巣上体炎","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Indoleamine 2,3-dioxygenase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Epididymitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Indoleamine 2,3-dioxygenase","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"精巣上体炎におけるindoleamine 2,3-dioxygenase（IDO）の役割に関する検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"精巣上体炎におけるindoleamine 2,3-dioxygenase（IDO）の役割に関する検討","subitem_title_language":"ja"},{"subitem_title":"The role of indoleamine 2,3-dioxygenase (IDO) in epididymitis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["617"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-03-14"},"publish_date":"2019-03-14","publish_status":"0","recid":"1915","relation_version_is_last":true,"title":["精巣上体炎におけるindoleamine 2,3-dioxygenase（IDO）の役割に関する検討"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2024-04-12T07:31:07.513776+00:00"}