{"created":"2023-06-19T10:29:50.185344+00:00","id":1939,"links":{},"metadata":{"_buckets":{"deposit":"b65f518d-ea1f-4fa6-baab-3cc7f29e7a8a"},"_deposit":{"created_by":31,"id":"1939","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"1939"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00001939","sets":["1709617079800:35:616:623"]},"author_link":["110379","110380"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"149","bibliographicPageStart":"137","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_10001_description_33":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著論文","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10001_description_34":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Regular Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"川崎医学会","subitem_publisher_language":"ja"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-J44(2)137","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2018/KMJ-J44(2)137.pdf","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_10001_text_31":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学泌尿器科学"}]},"item_10001_text_32":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Urology, Kawasaki Medical School"}]},"item_10001_textarea_5":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"　Lower urinary tract symptoms (LUTS) are considered to be caused by vascular insufficiency to bladder, and include voiding, urine collection, and post-voiding symptoms. The bladder outlet obstruction (BOO) model of rat is used as a pathological model, in which ischemia of the bowel is induced by obstruction, followed by chronic ischemia of the bowel and oxidant stress, resulting in LUTS. Since the antioxidant and anti-apoptotic actions of hydrogen have been attracting attention in recent years, it has been clarified that hydrogen could protect various organs, and multiple facilities and fields have reported that hydrogen could be used in the prevention and treatment of various diseases. In this study, we administered hydrogen water (H2) to the BOO model rats to investigate its effects and mechanism of action.\n　We used 9-week old female rats to prepare the BOO model. After laparotomy, a 19G needle was placed along the urethra and sutured to the urethra to induce partial obstruction of the urethra. Immediately after the preparation, the rats were divided into the sham, hydrogen water no-administration (BOO H2(－)), and administration (BOO H2(＋)) groups to compare the effects of intravesical pressure, and the histological, biochemical and immunohistological changes in 4 weeks.\n　The weight of the urinary bladder increased significantly in the BOO groups, 3.0- and 2.3- fold in the BOO H2(－) and BOO H2(＋) groups, respectively, compared with the sham group. The urination volume in one voiding increased significantly in the BOO groups, compared with the sham group. In the measurement of intravesical pressure, hyperreflexia of the urinary bladder was confirmed in the BOO groups, and the degree of detrusor overactivity was increase significantly in the BOO H2(－) group, compared with the BOO H2(＋) group. The proportion of collagen fiber in the bladder musculature increased significantly in the BOO H2(－) group, compared with the sham and BOO H2(＋) groups. In the quantification of 8-OHdG in the bladder tissues, the levels in the BOO H2(－) group were higher than the BOO H2(＋) group. In the 8-OHdG staining, significant expression was observed in the muscle layers only in the BOO H2(－) group. In the exhaustive chemokine/cytokine analysis, TNF-α was obviously inhibited in the comparison between the BOO H2(－) and BOO H2(＋) groups. In fact, TNF-α immune tissue imaging, expression was increased in the interstitium beneath the bladder cell mucosa and the muscle layers of the BOO H2(－) group. In the BOO H2(＋) group, TNF-α expression was reduced in the muscle layers.\n　Hydrogen water would be useful treatment as new alternative therapy for LUTS caused by the ischemic change of bladder. In this study, one of the mechanism of action of hydrogen water should be inhibition of various cytokines with a focus on TNF-α via inhibition of the oxidant stress."}]},"item_10001_textarea_6":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"　下部尿路症状（low urinary tract symptoms: LUTS）は，動脈硬化や前立腺肥大症に伴う下部尿路閉塞による膀胱血流障害が原因の一つとして考えられており，症状として排尿症状，畜尿症状，排尿後症状がある．ラットの下部尿路閉塞（bladder outlet obstruction: BOO）モデルは閉塞に伴い膀胱虚血を生じさせ，酸化ストレス状態を惹起し，LUTS を引き起こす病態モデルとして確立されている．近年，水素がもつ抗酸化，抗アポトーシス作用が注目されており，様々な臓器において組織の保護作用を示すことが解明され，様々な疾患の予防と治療に応用できることが多施設，多領域から報告されている．今回，ラットBOO モデルに対して，水素水（Hydrogen Water: H2）投与を行い，抗酸化作用の検証に加え，水素水が影響を及ぼす代表的メディエーターの探索を行った．\n　BOO モデルの作成には，９週齢の雌性ラットを用いた．開腹し，尿道に19G 針を沿わせた状態で尿道を結紮し，尿道の部分閉塞を作成した．作成直後からsham群，水素水非投与（BOO H（2 －））群と投与（BOO H（2 ＋））群に分け，４週間後，膀胱機能検査と組織学的，生化学的および免疫組織学的検討を行った．なお，水素水は経口投与した．\n　膀胱重量および一回排尿量は，sham 群に対して BOO H（2 ＋）群で有意差はなかったが，BOO H（2 －）群で有意に増加していた（重量 p<0.01），（ 一回排尿量 p<0.05）．BOO H（2 －）群と BOO H（2 ＋）群では有意差は認めなかった（p>0.05）．膀胱内圧測定では，BOO 群で排尿筋過活動が確認され，BOO H（2 －）群では，BOO H（2 ＋）群と比較して，排尿筋過活動回数は有意に増加していた（p<0.01）．膀胱筋層部における膠原繊維の比率については，BOO H2 （−）群でsham 群および BOO H（2 ＋）群に比して，有意に上昇していた（vs sham p<0.01, vs BOO H（2 ＋） p<0.01）．膀胱組織内8-OHdG の定量では，BOO H（2 －）群は BOO H2（＋）群に比して高値を示しており（p<0.05），8-OHdG 染色ではBOO H2 （－）群のみ筋層で著明な発現が認められた．網羅的ケモカイン/サイトカイン解析ではTNF-αが，BOO H（2 －）群においてBOO H（2 ＋）群と比較してもっとも発現が亢進しており，免疫染色では，BOO H2 （－）群で膀胱上皮粘膜下の間質および筋層においての発現の増加が認められた．一方，BOO H（2 ＋）群では，筋層部においての発現が抑制されていた．\n　水素水は，慢性虚血に起因する下部尿路症状の治療の一つになり得る可能性がある．本研究において，膀胱筋層の酸化ストレスを抑制することにより，膀胱筋層のTNF-α を中心とした炎症性サイトカインの抑制が，その作用機序の一つとして考えられた．"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"清水, 真次朗","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIMIZU, Shinjiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-04"}],"displaytype":"detail","filename":"KMJ-J44(2)137.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/1939/files/KMJ-J44(2)137.pdf"},"version_id":"a8896ae5-6e36-405d-aaad-35349b54abc5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"下部尿路閉塞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"下部尿路症状","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"水素水","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"酸化ストレス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"bladder outlet obstruction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"low urinary tract symptoms","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrogen water","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oxidative stress","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"水素水はラット下部尿路閉塞モデルにおける膀胱機能改善効果を示す","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"水素水はラット下部尿路閉塞モデルにおける膀胱機能改善効果を示す","subitem_title_language":"ja"},{"subitem_title":"Hydrogen water shows the effect of improving bladder function in a rat model of bladder outlet obstruction","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["623"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-04-04"},"publish_date":"2019-04-04","publish_status":"0","recid":"1939","relation_version_is_last":true,"title":["水素水はラット下部尿路閉塞モデルにおける膀胱機能改善効果を示す"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2024-04-13T02:55:17.553608+00:00"}