{"created":"2023-06-19T10:28:06.687209+00:00","id":274,"links":{},"metadata":{"_buckets":{"deposit":"e48da787-f739-4e6f-829f-979420e33f34"},"_deposit":{"created_by":31,"id":"274","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"274"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00000274","sets":["1709617079800:37:115:362"]},"author_link":["126612","126613"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"58","bibliographicPageStart":"53","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1989en/15(2)53-58.1989.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-E15(2)53 ","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00710169","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12029005","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0234","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2434-3404","subitem_source_identifier_type":"EISSN"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Kawasaki Medical School"}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Chemically purified abnormal peptides, α62-76 of Hb Ube-2, βT-3 of Hb G-Coushatta, αT-7 of Hb J-Norfolk and βT-1 of Hb Himeji, were subjected to molecular secondary ion mass spectrometry (SIMS). These peptides were identified by the mass number of their protonated molecular ion (M+H)+ and their amino acid sequence was confirmed by a series of the mass number of fragmented ions released by a stepwise removal from their C-terminus. Amino acid substitution of α68 Asn→Asp in Hb Ube-2, β22 Gln→ Ala in Hb G-Coushatta and α51 Gly→Asp in Hb J-Norfolk was successfully determined and a questionable Hb variant, tentatively called Hb Himeji (β140 Ala→ Asp) was proven to have a significant amount of the additive of the glycated N-terminus of the βT-1 peptide. "}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HIDAKA, Kazuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"126612","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IUCHI, Iwao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"126613","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00010214039.pdf","filesize":[{"value":"480.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00010214039.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/274/files/KJ00010214039.pdf"},"version_id":"91a9d9bd-b43e-454d-88c2-b6edd2f356e6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Secondary ion mass spectrometry -SIMS-","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hb Ube-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hb G-Coushatta","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hb Himeji","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Structural Analysis of Human Hemoglobin Variants by Molecular Secondary Ion Mass Spectrometry","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Structural Analysis of Human Hemoglobin Variants by Molecular Secondary Ion Mass Spectrometry","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["362"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"274","relation_version_is_last":true,"title":["Structural Analysis of Human Hemoglobin Variants by Molecular Secondary Ion Mass Spectrometry"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2023-09-26T10:28:27.868395+00:00"}