{"created":"2023-06-19T10:30:49.286669+00:00","id":2972,"links":{},"metadata":{"_buckets":{"deposit":"56a395ca-cff4-4b49-8eef-ad93495ded05"},"_deposit":{"created_by":3,"id":"2972","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2972"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00002972","sets":["1709617079800:37:801"]},"author_link":["130920","130919","130923","130921","130922","130924","130925","130918"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"19","bibliographicPageStart":"13","bibliographicVolumeNumber":"47","bibliographic_titles":[{},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_10001_description_34":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Regular Article","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Kawasaki Medical Society"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-E202147013","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2021en/KMJ-E202147013.pdf","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12685295","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12029005","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03850234","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"24343404","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Laboratory of Microbiology, Showa Pharmaceutical University"},{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":" Second Year Medical Student in fiscal year of 2020, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":" Second Year Medical Student in fiscal year of 2020, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Kawasaki Medical School"}]},"item_10001_textarea_5":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"　Transmembrane proteins play essential roles in cell signaling, transport of membrane-impermeable molecules, cell-cell communication, and cell adhesion. Our recent work demonstrated that reactive oxygen species-generating NADPH oxidase 4 (Nox4), a protein with multiple transmembrane domains, is involved in cell migration by stabilizing vascular endothelial growth factor receptor 2 (VEGFR-2), a single-span transmembrane protein. During this study and with further verification, we developed a simple method to prepare protein samples without aggregating these membrane proteins for SDS-PAGE, immunoblotting, and deglycosylation assay. We found that heating was unnecessary for protein denaturation for SDS-PAGE and deglycosylation assay. Also, the detectable amounts of VEGFR-2 and Nox4 were increased in the sample treated at 4℃ compared with the sample treated at 98℃ . Moreover, the N-glycan of VEGFR-2 was digested by glycosidase at reaction temperature 4℃ ."}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MIYANO, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OKAMOTO, Shuichiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAJIKAWA, Mizuho","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAWAI, Chikage","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KANAGAWA, Tomoka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TOMINAGA, Sayuri","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAUCHI, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KURIBAYASHI, Futoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-01"}],"displaytype":"detail","filename":"KMJ-E202147013.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmed.repo.nii.ac.jp/record/2972/files/KMJ-E202147013.pdf"},"version_id":"a9cb057d-97a6-4b89-88ae-d183ab4f62c2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Membrane protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endoglycosidase, Immunoblotting","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SDS-PAGE, VEGFR-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NADPH oxidase 4","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The efficient detection of membrane protein with immunoblotting: lessons from cold-temperature denaturation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The efficient detection of membrane protein with immunoblotting: lessons from cold-temperature denaturation","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["801"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-01"},"publish_date":"2022-04-01","publish_status":"0","recid":"2972","relation_version_is_last":true,"title":["The efficient detection of membrane protein with immunoblotting: lessons from cold-temperature denaturation"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T10:50:14.263690+00:00"}