| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-03-10 |
| タイトル |
|
|
タイトル |
Diagnostic accuracy of 16S ribosomal RNA gene polymerase chain reaction in bacteremia: A prospective observational study |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
TAKAHASHI, Jiro
MIYATA, Ippei
INOUE, Takahiro
MIYAMOTO, Satomi
YAMADA, Sachiko
UENO, Daisuke
INAYOSHI, Yuki
OUCHI, Kazunobu
SHIINO, Yasukazu \n\n
|
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Acute Medicine |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pediatrics, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Acute Medicine |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Acute Medicine |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Acute Medicine |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Acute Medicine |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Acute Medicine |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Medical Welfare for Children, Faculty of Health and Welfare, Kawasaki University of Medical Welfare |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Acute Medicine |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Real-time PCR |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bacteremia |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Early diagnosis |
| 抄録(英) |
|
|
言語 |
en |
|
値 |
The standard method for diagnosing bacteremia is blood culture. However, the sensitivity of blood culture is low when the number of bacteria in the blood is low or when antibiotics have already been administered. Furthermore, some bacteria are difficult to detect in blood cultures. 16S ribosomal RNA (rRNA) contains conserved sequences that are targeted for PCR amplification using universal primers. We investigated whether the threshold cycle (Ct) value of 16S rRNA real-time PCR in whole-blood samples can be used for early diagnosis of bacteremia. Ct values of the 16S rRNA real-time PCR in 307 collected specimens showed a bimodal distribution. Ct values of the blood culture-positive group were significantly lower than those of the blood culture-negative group (P < 0.001). The cutoff value of the receiver operating characteristic curve was 38.80, as determined using finite-mixture modeling and expectation-maximization algorithm. Analysis of the diagnostic accuracy at this cutoff value showed a sensitivity of 91.4%, specificity of 33.5%, positive predictive value of 15.0%, and negative predictive value of 96.8%. The Ct value of 16S rRNA real-time PCR shows high negative predictive value, it may be useful for excluding bacteremia when the cutoff value is set appropriately. |
| 書誌情報 |
en : Kawasaki medical journal
巻 48,
p. 61-71,
発行日 2022
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| 出版者 |
|
|
出版者 |
Kawasaki Medical Society |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
24343404 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/KMJ-E202248061 |
| 記事種別(英) |
|
|
内容記述 |
Regular Article |
|
言語 |
en |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2022en/KMJ-E202248061.pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
PDF (1.3 MB)
