{"created":"2023-06-19T10:28:10.628566+00:00","id":330,"links":{},"metadata":{"_buckets":{"deposit":"3db49be1-ca55-4a4d-b822-5b2423b4e6e3"},"_deposit":{"created_by":31,"id":"330","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"330"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00000330","sets":["1709617079800:37:49:371"]},"author_link":[],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1･2","bibliographicPageEnd":"24","bibliographicPageStart":"11","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1992en/18(1.2)11-24.1992.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ1992en/18(1.2)11-24.1992.pdf","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00710169","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12029005","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0234","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2434-3404","subitem_source_identifier_type":"EISSN"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Anatomy, Kawasaki Medical School"}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Glycoconjugates on the cell surface of type I and type II pulmonary epithelial cells were examined after airway irrigation by means of colloidal iron (CI), high iron diamine (HID) and ferritin-conjugated lectin stainings, in combination with digestion with carbohydrate-degrading enzymes, methylation or extraction with acid ethanol. The acid saccharide on the surface of type I cells was sialic acid, but the surface of type II cells contained a mixture of a large amount of sialic acid and a small amount of sulfated saccharide. As to the sensitivity on acid ethanol extraction, the sialo-oligosaccharide on type I cells differed from that on type II cells, although the terminal trisaccharides of sialo-oligosaccharides on the both type I and II cells were sialic acid → galactose → N-acetylgalactosamine. Sulfated saccharide, being mainly composed of glucuronic acid and N-acetylgalactosamine, distributed on the surface of the microvilli of type II cells. As to the constituents of glycoconjugates, large amounts of N-acetylglucosamine, galactose, mannose and glucose were found on type I cells, but type II cells had only small amounts of N-acetylglucosamine, mannose and glucose, and a minute amount of galactose. "}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"IWATSUKI, Hirohiko","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00010202479.pdf","filesize":[{"value":"6.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00010202479.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/330/files/KJ00010202479.pdf"},"version_id":"28b95977-2fa8-4597-bef8-fa377b0cc5fa"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Glycoconjugates","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lung","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pulmonary epithelial cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell membrane","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Histochemistry","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Glycoconjugates on the Surface of the Pulmonary Epithelial Cells in Surfactant Free Rat Lungs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Glycoconjugates on the Surface of the Pulmonary Epithelial Cells in Surfactant Free Rat Lungs","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["371"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"330","relation_version_is_last":true,"title":["Glycoconjugates on the Surface of the Pulmonary Epithelial Cells in Surfactant Free Rat Lungs"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2023-09-29T02:09:40.890875+00:00"}