{"created":"2023-06-19T10:28:26.789014+00:00","id":588,"links":{},"metadata":{"_buckets":{"deposit":"204a2205-130d-4a18-bb0a-decea80481f5"},"_deposit":{"created_by":31,"id":"588","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"588"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00000588","sets":["1709617079800:37:41:426"]},"author_link":["125325","125326","125327","125328","125329","125330"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"29","bibliographicPageStart":"25","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Regular Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2016en/KMJ-E42(1)25.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-E42(1)25","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA12685295","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12029005","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0234","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2434-3404","subitem_source_identifier_type":"EISSN"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Public Health, Kawasaki Medical School・Department of Bacteriology II, National Institute of Infectious Diseases・Department of Infectious Diseases, Yonago Medical Center, National Hospital Organization"},{"subitem_text_language":"en","subitem_text_value":"Department of Bacteriology II, National Institute of Infectious Diseases"},{"subitem_text_language":"en","subitem_text_value":"Department of Bacteriology II, National Institute of Infectious Diseases"},{"subitem_text_language":"en","subitem_text_value":"Department of Bacteriology II, National Institute of Infectious Diseases"},{"subitem_text_language":"en","subitem_text_value":"Department of Public Health, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Department of Public Health, Kawasaki Medical School"}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Although fluoroquinolone (FQ) has been used for the treatment of various bacterial infectious diseases, its continued use has been problematic given the appearance of FQ-resistant bacteria. However, the recent discovery of four plasmid-mediated quinolone resistance (PMQR) genes comprising qnr, aac(6')Ib-cr, qepA and OqxAB since 1998 has provided insights in the area of FQ-resistance. For practical detection of qepA in microbiology laboratory, a specific, simple, rapid and cost-effective isothermal amplification method designated as LAMP is the good candidate to use. In this study, the development of a new detection method using LAMP to identify qepA1, one variant of the qepA gene, was tried. As the results, the LAMP method using a qepA1-specific LAMP primer set comprising five primerscould detect all four qepA1-positive strains in addition to 17 qepA1-negative strains. The LAMP method is clearly much more advantageous for use in clinical laboratories. Furthermore, the time and accuracy benefits allow for the selection of antibiotics in a clinical setting."}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMANE, Kunikazu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HORINO, Atsuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Satowa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIBAYAMA, Keigo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUSHIMI, Shigeko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KATSUYAMA, Hironobu","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00010242639.pdf","filesize":[{"value":"690.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00010242639.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/588/files/KJ00010242639.pdf"},"version_id":"10d5904d-41c2-4bc2-90e8-f448d0a5ae00"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"QepA1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmid-mediated Quinolone Resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"LAMP","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Simple and rapid detection method for qepA1 by loop-mediated isothermal amplification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Simple and rapid detection method for qepA1 by loop-mediated isothermal amplification","subitem_title_language":"en"}]},"item_type_id":"3","owner":"31","path":["426"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"588","relation_version_is_last":true,"title":["Simple and rapid detection method for qepA1 by loop-mediated isothermal amplification"],"weko_creator_id":"31","weko_shared_id":-1},"updated":"2023-10-10T06:59:30.371683+00:00"}