{"created":"2023-06-19T10:28:29.054168+00:00","id":621,"links":{},"metadata":{"_buckets":{"deposit":"6221ba4a-7bf6-4d54-b890-128d3d06567b"},"_deposit":{"created_by":25,"id":"621","owners":[25],"pid":{"revision_id":0,"type":"depid","value":"621"},"status":"published"},"_oai":{"id":"oai:kwmed.repo.nii.ac.jp:00000621","sets":["1709617079800:35:186:431"]},"author_link":["120234","120235"],"item_1694495855422":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"302","bibliographicPageStart":"295","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"川崎医学会誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Kawasaki medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_identifier_14":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1987/KMJ13(3)295-302.1987.pdf"}]},"item_3_relation_20":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11482/KMJ-J13(3)295 ","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00045593","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AN12940574","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-5924","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2758-089X","subitem_source_identifier_type":"EISSN"}]},"item_3_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"川崎医科大学泌尿器科"}]},"item_3_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Urology, Kawasaki Medical School"}]},"item_3_textarea_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"高速液体クロマトグラフィー(HPLC)を用いて,副腎・精巣ステロイド分析の予備的検討を行った.使用したHPLCシステムはLC 5A型(Shimazu)でUV detectorとcosmosil packed column 5C18を接続した.progesterone,deoxycorticosterone,corticosterone,cortisol,testosteroneの5種類のステロイドは明瞭に分離可能であり,直線の検量線を得ることができた.しかし近似したretention timeのステロイドの分離は困難であった.平均回収率は93.0%であり,このクロマトグラフィーシステムの限界感度はngオーダーであった.HPLCはヒト副腎および精巣ホモジネート組織,ならびにラット副腎細胞短期間培養浮遊液より抽出したステロイドの分離・同定・定量に有用であることが示唆された."}]},"item_3_textarea_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"The analysis of adrenal and testicular steroids was preliminarily investigated using a high performance liquid chromatography (HPLC). This system was LC-5A type (Shimazu) and equipped with a UV detector and cosmosil packed column 5C18. The best condition for the separation of mixed steroids was investigated. Five steroids (progesterone, deoxycorticosterone, corticosterone, cortisol, testosterone) were sharply separated and obtained a linear calibration curve. Separations of similar retention time steroids were difficult. Mean percentage recovery was 93.0%. Sensitivity of the steroid determination was ng order at this chromatographic system. HPLC was a useful tool for separation, identification, and quantitation of steroids that extracted from homogenized tissue of adrenal gland and testicular tissue in human and isolated adrenal cells of rat, too."}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡辺, 慶太","creatorNameLang":"ja"},{"creatorName":"ワタナベ, ケイタ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"120234","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Keita","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"120235","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"KJ00009816859.pdf","filesize":[{"value":"735.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00009816859.pdf","objectType":"fulltext","url":"https://kwmed.repo.nii.ac.jp/record/621/files/KJ00009816859.pdf"},"version_id":"c9794ceb-d2dc-4c65-b482-58deee02efe8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"High performance liquid chromatography (HPLC)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Analysis of adrenal and testicular steroids","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"高速液体クロマトグラフィーによる副腎・精巣ステロイドの分析 : △^4ステロイドを中心に","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高速液体クロマトグラフィーによる副腎・精巣ステロイドの分析 : △^4ステロイドを中心に","subitem_title_language":"ja"},{"subitem_title":"Analysis of Adrenal and Testicular Steroids by High Performance Liquid Chromatography : A Preliminary Report of Determinations of △^4-Steroids","subitem_title_language":"en"}]},"item_type_id":"3","owner":"25","path":["431"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"621","relation_version_is_last":true,"title":["高速液体クロマトグラフィーによる副腎・精巣ステロイドの分析 : △^4ステロイドを中心に"],"weko_creator_id":"25","weko_shared_id":-1},"updated":"2023-10-06T04:23:14.951449+00:00"}