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TNF receptor type 2 transmits caspase-dependent apoptotic signals in fibroblast-like synoviocytes derived from rheumatoid arthritis
https://kwmed.repo.nii.ac.jp/records/580
https://kwmed.repo.nii.ac.jp/records/580a6eb4089-1b61-4aa7-b2f0-61dad276603e
名前 / ファイル | ライセンス | アクション |
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KJ00010041779.pdf (1.5 MB)
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Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-01-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | TNF receptor type 2 transmits caspase-dependent apoptotic signals in fibroblast-like synoviocytes derived from rheumatoid arthritis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hirano, Hiroyasu
× Hirano, Hiroyasu× Igarashi, Hideya× Morita, Yoshitaka× Ishihara, Katsuhiko |
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著者所属(英) | ||||||
en | ||||||
Department of Immunology and Molecular Genetics Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Immunology and Molecular Genetics Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Rheumatology Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Immunology and Molecular Genetics Kawasaki Medical School | ||||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Rheumatoid arthritis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Fibroblast-like synoviocyte | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Apoptosis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Caspase | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Original Article | |||||
言語 | en | |||||
抄録(英) | ||||||
en | ||||||
Signals from tumor necrosis factor α (TNFα) are transduced through two types of receptors, tumor necrosis factor receptor type1 (TNFR1) and type2 (TNFR2), which commonly transduce activation signals for NF-κB, affecting cellular survival, growth, and inflammation. TNFR1 is ubiquitously expressed and mediates caspase-dependent apoptotic signals, whereas TNFR2 is selectively expressed on hematopoietic cells without transducing death signals. We detected TNFR2 transcription in fibroblast-like synoviocytes derived from rheumatoid arthritis (RA-FLS) at various levels, but usually much lower than those of TNFR1. To investigate the function of TNFR2 on RA-FLS in TNFα signaling, we established a stable transfectant overexpressing TNFR2 using the human RA-FLS cell line MH7A and stimulated it with 50ng/ml TNFα, a concentration that usually induces apoptosis in parent MH7A cells. Since TNFR2 is known to transduce anti-apoptotic signals via NF-κB activation, we expected to observe a reduction in apoptotic cells. Contrary to our expectations, the ratio of apoptotic cells in TNFR2 transfectants was higher than that of mock stable transfectants used as a control. This enhanced sensitivity to apoptosis was not inhibited by the addition of either anti-TNFR2 monoclonal antibody (mAb) 80M2, which blocks ligand passing, or antagonistic anti-TNFR1 Ab, indicating that apoptosis was independent of TNFR1 signals. Furthermore, in the presence of antagonistic anti-TNFR1 Ab, the addition of agonistic anti-TNFR2 Ab induced apoptosis with a rapid decrease in TNF receptor-associated factor 2 (TRAF2) and cleavage of caspase-8and-3. The observed apoptosis was sensitive to an inhibitor of pan-caspase, but not of receptor-interacting protein (RIP) 1. These data clearly indicate the presence of a caspasedependent, apoptotic signaling pathway downstream of TNFR2. | ||||||
書誌情報 |
en : Kawasaki medical journal 巻 41, 号 2, p. 29-40, 発行日 2015 |
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URL | ||||||
識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2015en/KMJ-E41(2)29.pdf | |||||
識別子タイプ | URI | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11482/KMJ-E41(2)29 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0385-0234 | |||||
ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2434-3404 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12685295 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12029005 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |