| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
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|
タイトル |
TNF receptor type 2 transmits caspase-dependent apoptotic signals in fibroblast-like synoviocytes derived from rheumatoid arthritis |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Hirano, Hiroyasu
Igarashi, Hideya
Morita, Yoshitaka
Ishihara, Katsuhiko
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| 著者所属(英) |
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en |
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Department of Immunology and Molecular Genetics Kawasaki Medical School |
| 著者所属(英) |
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en |
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Department of Immunology and Molecular Genetics Kawasaki Medical School |
| 著者所属(英) |
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en |
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Department of Rheumatology Kawasaki Medical School |
| 著者所属(英) |
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en |
|
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Department of Immunology and Molecular Genetics Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
Rheumatoid arthritis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Fibroblast-like synoviocyte |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Apoptosis |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Caspase |
| 記事種別(英) |
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内容記述タイプ |
Other |
|
内容記述 |
Original Article |
|
言語 |
en |
| 抄録(英) |
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en |
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Signals from tumor necrosis factor α (TNFα) are transduced through two types of receptors, tumor necrosis factor receptor type1 (TNFR1) and type2 (TNFR2), which commonly transduce activation signals for NF-κB, affecting cellular survival, growth, and inflammation. TNFR1 is ubiquitously expressed and mediates caspase-dependent apoptotic signals, whereas TNFR2 is selectively expressed on hematopoietic cells without transducing death signals. We detected TNFR2 transcription in fibroblast-like synoviocytes derived from rheumatoid arthritis (RA-FLS) at various levels, but usually much lower than those of TNFR1. To investigate the function of TNFR2 on RA-FLS in TNFα signaling, we established a stable transfectant overexpressing TNFR2 using the human RA-FLS cell line MH7A and stimulated it with 50ng/ml TNFα, a concentration that usually induces apoptosis in parent MH7A cells. Since TNFR2 is known to transduce anti-apoptotic signals via NF-κB activation, we expected to observe a reduction in apoptotic cells. Contrary to our expectations, the ratio of apoptotic cells in TNFR2 transfectants was higher than that of mock stable transfectants used as a control. This enhanced sensitivity to apoptosis was not inhibited by the addition of either anti-TNFR2 monoclonal antibody (mAb) 80M2, which blocks ligand passing, or antagonistic anti-TNFR1 Ab, indicating that apoptosis was independent of TNFR1 signals. Furthermore, in the presence of antagonistic anti-TNFR1 Ab, the addition of agonistic anti-TNFR2 Ab induced apoptosis with a rapid decrease in TNF receptor-associated factor 2 (TRAF2) and cleavage of caspase-8and-3. The observed apoptosis was sensitive to an inhibitor of pan-caspase, but not of receptor-interacting protein (RIP) 1. These data clearly indicate the presence of a caspasedependent, apoptotic signaling pathway downstream of TNFR2. |
| 書誌情報 |
en : Kawasaki medical journal
巻 41,
号 2,
p. 29-40,
発行日 2015
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| URL |
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|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2015en/KMJ-E41(2)29.pdf |
|
識別子タイプ |
URI |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.11482/KMJ-E41(2)29 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0385-0234 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2434-3404 |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12685295 |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| 著者版フラグ |
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出版タイプ |
VoR |
KJ00010041779.pdf (1.5 MB)
