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Detection of the Hb E andβ^E-globin genes was carried out by DEAE-HPLC and isoelectric focusing of the hemolysates, by electrophoresis of DNA fragments which resulted in the digestion of the PCR product with Mn/I, and by PCR-direct sequencing. The haplotype of the β-globin-like gene cluster was determined by the presence (+) or absence (-) of the restriction enzyme site in the PCR product including the polymorphic site. Polymorphism or the haplotype were determined at seven sites, 5\u0027 to the ε-gene/HincII, in the ^Gγ-gene/HindIII, in the ^Aγ-gene/HindIII, in the ψβ-gene/HincII, 3\u0027 to the ψβ-gene/HincII, in the β-IVS II/AvaII and 3\u0027 to the β-gene/BamHI, in the β-globin-like gene cluster. Three types of haplotypes were found in this study; (a) - + - + + + - (five families), (b) + - - - - + - (four families) and (c) + - - + + + - (one family). Haplotypes (a) and (b) were identified as one\u0027s in the Thai and Cambodian populations in eastern Myanmar. They were considered to be the most common haplotypes in that area and to have been the result of gene confusion or gene flow between ethnic groups. 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Haplotypes of the β^E-Globin Gene Cluster Found in Myanmar
https://kwmed.repo.nii.ac.jp/records/528
https://kwmed.repo.nii.ac.jp/records/528e46c0b1f-9e4e-4039-be89-b5d53ed3fcae
名前 / ファイル | ライセンス | アクション |
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KJ00000010576.pdf (1.2 MB)
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Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-01-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Haplotypes of the β^E-Globin Gene Cluster Found in Myanmar | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
HARANO, Keiko
× HARANO, Keiko× Tin, Yi Yi× HARANO, Teruo |
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著者所属(英) | ||||||
en | ||||||
Department of Clinical Nutrition, Faculty of Medical Professions,Kawasaki University of Medical Welfare | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Biochemistry, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Biochemistry, Kawasaki Medical School | ||||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | hemoglobin E (Hb E) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | haplotypes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | PCR-restriction enzyme | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | PCR-direct sequencing | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Myanmar | |||||
抄録(英) | ||||||
en | ||||||
We had the opportunity to study the members of 10 families with Hb E/β-thalassemia and characterized the ethnic features of the population of Myanmar from haplotyping of aβ-globin-like gene cluster producing Hb E. Detection of the Hb E andβ^E-globin genes was carried out by DEAE-HPLC and isoelectric focusing of the hemolysates, by electrophoresis of DNA fragments which resulted in the digestion of the PCR product with Mn/I, and by PCR-direct sequencing. The haplotype of the β-globin-like gene cluster was determined by the presence (+) or absence (-) of the restriction enzyme site in the PCR product including the polymorphic site. Polymorphism or the haplotype were determined at seven sites, 5' to the ε-gene/HincII, in the ^Gγ-gene/HindIII, in the ^Aγ-gene/HindIII, in the ψβ-gene/HincII, 3' to the ψβ-gene/HincII, in the β-IVS II/AvaII and 3' to the β-gene/BamHI, in the β-globin-like gene cluster. Three types of haplotypes were found in this study; (a) - + - + + + - (five families), (b) + - - - - + - (four families) and (c) + - - + + + - (one family). Haplotypes (a) and (b) were identified as one's in the Thai and Cambodian populations in eastern Myanmar. They were considered to be the most common haplotypes in that area and to have been the result of gene confusion or gene flow between ethnic groups. Haplotype (c), on the other hand, was a rare type, suggesting both the specificity and diversity of ethnic groups in Myanmar. | ||||||
書誌情報 |
en : Kawasaki medical journal 巻 29, 号 1・2, p. 9-16, 発行日 2003 |
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URL | ||||||
識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2003en/29(1.2)9-16.2003.PDF.pdf | |||||
識別子タイプ | URI | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11482/KMJ29(1.2)9-16.2003.PDF.pdf | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0385-0234 | |||||
ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2434-3404 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00710169 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12029005 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |