| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
|
|
タイトル |
Establishment of an asthma model by sensitization with mite antigen alone in C57BL/6J mice |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Shimizu, Hiroki
Obase, Yasushi
Katoh, Shigeki
Ikeda, Masaki
Mouri, Keiji
Kobashi, Yoshihiro
Oka, Mikio
|
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Asthma |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mite Extract-Dermatophagoides farina |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cytokine |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Chemokine |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Asthma model mice |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
C57BL,6J |
| 記事種別(日) |
|
|
内容記述タイプ |
Other |
|
内容記述 |
原著 |
|
言語 |
ja |
| 抄録(英) |
|
|
|
en |
|
|
Bronchial asthma is characterized by the bronchial hyperresponsiveness and airway obstruction related to airway smooth muscle contraction. Eosinophilic airway inflammation is involved in its pathogenesis. To reproduce the condition, various animal models have been prepared. However, there are many models that do not reflect the spontaneous history of bronchial asthma onset in humans due to the mouse strain, sensitizing antigen, or administration method. In this study, we prepared a mouse model of which the mechanism is similar to that of human bronchial asthma. Mite Extract-Dermatophagoides farinae (Derf) antigen was transnasally administered to wild-type C57BL/6J mice (WT) 13 times. Subsequently, an airway hypersensitivity test (Mch PC_200), specific antigen exposure test (ΔSRaw), bronchoalveolar lavage (BAL), and blood collection were performed to examine the presence or absence of asthma acquisition and differences in the local pulmonary levels of cytokines/chemokines in comparison with the physiological saline-treated group. In the mite antigen-treated mice (WT/-Derf), bronchial hyperresponsiveness was enhanced, antigen-specific was increased airway resistance in comparison with physiological salinetreated mice (WT/-Saline). In addition, the number of eosinophils in BAL fluid (BALF) was greater. Furthermore, there was a correlation among leukotrienes, eotaxin, and tissue inhibitors of metalloproteinase 1 in BALF, suggesting that the mechanism concerning eosinophilic airway inflammation involving in human bronchial asthma was reproduced. In this study, we successfully established a mouse bronchial asthma model in which the pathogenesis resembles that in humans in comparison with conventional models, using Derf antigen alone and C57BL/6J mice. |
| 書誌情報 |
ja : 川崎医学会誌
en : Kawasaki medical journal
巻 39,
号 1,
p. 13-19,
発行日 2013
|
| URL |
|
|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2013/KMJ31(1)13-19.2013.pdf |
|
識別子タイプ |
URI |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/2013/KMJ31(1)13-19.2013.pdf |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0386-5924 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2758-089X |
| 雑誌書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00045593 |
| 雑誌書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN12940574 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
KJ00008396899.pdf (269.8 kB)
