Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
公開日 |
2017-01-23 |
タイトル |
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タイトル |
Latency of Klebsiella species and Escherichia coli strains that harbor a conjugative IncA/C type plasmid carrying both rmtB and bla_<CTX-M-14> |
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言語 |
en |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
著者 |
Yamane, Kunikazu
Wachino, Junichi
Suzuki, Satowa
Arakawa, Yoshichika
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著者所属(英) |
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en |
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Department of Public Health, Kawasaki Medical School and Department of Bacteriology II, National Institute of Infectious Diseases |
著者所属(英) |
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en |
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Department of Bacteriology II, National Institute of Infectious Diseases and Department of Bacteriology, Nagoya University Graduate School of Medicine |
著者所属(英) |
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en |
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Department of Bacteriology II, National Institute of Infectious Diseases |
著者所属(英) |
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en |
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Department of Bacteriology II, National Institute of Infectious Diseases and Department of Bacteriology, Nagoya University Graduate School of Medicine |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Aminoglycoside resistance |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
16S rRNA methylase |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Enterobacteriacae |
記事種別(日) |
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内容記述タイプ |
Other |
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内容記述 |
原著 |
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言語 |
ja |
抄録(英) |
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en |
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Six high-level aminoglycoside and third generated cephalosporin of cefotaxime resistance Klebsiella pneumoniae, Klebsiella oxytoca, and Escherichia coli isolated from clinical specimens in the same Japanese hospital since 2001. These 6 strains were resistant to all 4,6-disubstituted deoxystreptamine including albekacin. The results of multiplex PCR for 16S rRNA methylase and bla_<TEM>, bla_<SHV>, bla_<CTX-M> specific PCR reaction show that these strains harbor 16S rRNA methylase of rmtB and β-lactamase resistance gene of bla_<CTX-M-9> group in the same large plasmid. Sequence analysis of bla_<CTX-M-9> group PCR products shows this β-lactamase is bla_<CTX-M-14>. rmtB-harbored plasmids are classified the IncA/C by PCR and restriction pattern by EcoRI show only one or two band differences. Moreover, southern hybridization of EcoRI digested rmtB harbored plasmid by rmtB probe show the same hybridization pattern of 4.8 kbp bands. PFGE fingerprinting analysis of four K. pneumoniae revealed two fingerprinting patterns. These facts suggested that rmtB-positive starains not only spread horizontal transfer of rmtB-harbored plasmid but also clonal spread of the same strain. |
書誌情報 |
ja : 川崎医学会誌
en : Kawasaki medical journal
巻 39,
号 3,
p. 87-94,
発行日 2013
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URL |
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識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2013/KMJ39(3)87-94.2013.pdf |
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識別子タイプ |
URI |
DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.11482/2013/KMJ39(3)87-94.2013.pdf |
ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0386-5924 |
ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2758-089X |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN00045593 |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN12940574 |
著者版フラグ |
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出版タイプ |
VoR |