| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
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タイトル |
Generation of retinal pigment epithelium from human induced pluripotent stem cells showed polarized secretion of VEGF and PEDF. |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kamao, Hiroyuki
Ishida, Junko
Suda, Taiji
Kiryu, Junichi
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| 著者所属(英) |
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en |
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Department of Ophthalmology, Kawasaki Medical School |
| 著者所属(英) |
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en |
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Department of Ophthalmology, Kawasaki Medical School |
| 著者所属(英) |
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en |
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Tissue Biology & Electron Microscopy Research Center, Kawasaki Medical School |
| 著者所属(英) |
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en |
|
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Department of Ophthalmology, Kawasaki Medical School |
| キーワード |
|
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Induced pluripotent stem cell |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Retinal pigment epithelium |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Regeneration therapy |
| 記事種別(日) |
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|
内容記述タイプ |
Other |
|
内容記述 |
原著 |
|
言語 |
ja |
| 抄録(英) |
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en |
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Age-related macular degeneration (AMD) is one of the major diseases that cause severe visual impairment in developed countries and is related to dysfunction of the retinal pigment epithelium (RPE). Human induced pluripotent stem cells (hiPSCs) have been drawing attention as a valuable source of RPE for basic research on or treatment of AMD. Here we investigated whether RPE could be generated from hiPSCs in Kawasaki Medical School. We maintained hiPSCs retaining pluripotent markers after a series of regular culture steps involving passaging, freezing, and thawing. hiPSCs were then cultured in RPE differentiation medium, and pigmented colonies were manually isolated for further differentiation into RPE. Differentiated cells formed pigmented cells with a typical RPE cobblestone appearance, abundant apical microvilli, adherens junctions, and tight junctions. Furthermore, generated pigmented cells expressed typical RPE marker genes, exhibited a barrier function, and secreted growth factors in a polarity-dependent manner similar to native RPE. These results indicate that RPE derived from hiPSCs in our facility can be used for in vitro and in vivo research. |
| 書誌情報 |
ja : 川崎医学会誌
en : Kawasaki medical journal
巻 39,
号 4,
p. 155-162,
発行日 2013
|
| URL |
|
|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2013/KMJ39(4)155-162.2013.pdf |
|
識別子タイプ |
URI |
| DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/2013/KMJ39(4)155-162.2013.pdf |
| ISSN |
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|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0386-5924 |
| ISSN |
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収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2758-089X |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00045593 |
| 雑誌書誌ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN12940574 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
KJ00008893739.pdf (537.5 kB)
