| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
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タイトル |
Differential Production of Proteolytic Enzymes by Normal (KMS-6), Immortally Transformed (KMST-6), and Tumorigenetically Transformed (Ha-KMST-6) Human Fibroblasts |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
NAMBA, Masayoshi
NISHITANI, Koji
KARAI, Machiko
KIMOTO, Tetsuo
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| 著者所属(英) |
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en |
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Department of Pathology, Kawasaki Medical School |
| 著者所属(英) |
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en |
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Department of Pathology, Kawasaki Medical School |
| 著者所属(英) |
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en |
|
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Department of Pathology, Kawasaki Medical School |
| 著者所属(英) |
|
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en |
|
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Department of Pathology, Kawasaki Medical School |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Human cells |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Transformation |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Proteases |
| 記事種別(英) |
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内容記述タイプ |
Other |
|
内容記述 |
Original Article |
|
言語 |
en |
| 抄録(英) |
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en |
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Production of proteolytic enzymes by human fibroblasts in the process of transformation was investigated in order to learn which step, the immortalizing or tumorigenic step, is more important for malignant transformation of human cells. The cells used were normal human fibroblasts (KMS-6), fibroblasts immortally transformed by treatment with Co-60 gamma rays (KMST-6), and KMST-6 cells further tumorigenetically transformed KMST-6 cells by infection with Harvey murine sarcoma viruses (Ha-KMST-6). Proteolytic enzymes in culture medium or cells were assayed using synthetic substrates, N-a-(p-tosyl)-L-arginine [3H]methyl ester hydrochloride and H-D-val-leu-lys-p-nitroaniline. Our results showed that the immortally transformed KMST-6 cells produced a larger amount of the enzymes than the normal and the tumorigenetically transformed cells. Since elevated production of proteolytic enzymes has been reported to correlate well with malignant transformation of cultured rodent or avian cells by many other investigators, our present results indicate that our immortalized human fibroblasts have already acquired properties characteristic to cancer cells. |
| 書誌情報 |
en : Kawasaki medical journal
巻 12,
号 1,
p. 15-22,
発行日 1986
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| URL |
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|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1986en/12(1)15-22.1986.pdf |
|
識別子タイプ |
URI |
| DOI |
|
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.11482/KMJ-E12(1)15 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0385-0234 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2434-3404 |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00710169 |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| 著者版フラグ |
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|
出版タイプ |
VoR |
KJ00010221419.pdf (819.0 kB)
