| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-03-18 |
| タイトル |
|
|
タイトル |
Novel analgesics targeting brain-derived neurotrophic factor for neuropathic pain |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
HAZAMA, Keita
IKEDA, Kae
TSUGE, Masatsugu
ITANO, Yoshitaro
OUCHIDA, Mamoru
NAKATSUKA, Hideki
|
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Anesthesiology and Intensive Care Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Anesthesiology and Intensive Care Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Anesthesiology and Intensive Care Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Anesthesiology and Intensive Care Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Molecular Oncology, Graduate School of Medicine,
Dentistry and Pharmaceutical Sciences, Okayama University |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Anesthesiology and Intensive Care Medicine, Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
neuropathic pain |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
BDNF |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
TrkB |
| 抄録(英) |
|
|
言語 |
en |
|
値 |
Brain-derived neurotrophic factor (BDNF) is necessary for the development, growth, and maintenance of nerve cells. BDNF is expressed in the dorsal root ganglion (DRG); binds to the Tropomyosin receptor kinasa B (TrkB) receptor, which has a tyrosine kinase domain, in the spinal cord; and plays an important role as a pain modulator. BDNF expression is increased in various types of pain, including acute pain, neuropathic pain, and cancer pain. Activation of the BDNF–TrkB pathway transmits pain information. In order to inhibit the BDNF–TrkB pathway, by sequestering BDNF, we constructed a cDNA expression plasmid encoding the extracellular region of rat TrkB fused to enhanced green fluorescent protein (EGFP). When the expression plasmid vector was administered to rat models of neuropathic pain, induced by spinal nerve ligation, statistically significant relief of pain was observed in terms of a 50% paw-withdrawal threshold using the von Frey test. The expression of TrkB-EGFP mRNA was detected in L5 lumbar vertebral nerves by quantitative reverse transcriptase polymerase chain reaction. To verify the pain-suppressive effect of the expression vector, truncated TrkB protein, without EGFP, was purified, and administered to pain model rats. A statistically significant suppressive effect of the truncated TrkB protein on neuropathic pain was observed 2 days after administration. The pain-suppressive effect of the truncated TrkB protein was more effective than that of the TrkB-Fc chimera protein and lasted longer than that of the TrkB antagonist ANA-12. Our results suggested that the truncated TrkB cDNA expression vector and truncated TrkB protein could be used as molecular targeted drugs in patients with neuropathic pain. |
| 書誌情報 |
en : Kawasaki medical journal
巻 44,
号 2,
p. 41-52,
発行日 2018
|
| 出版者 |
|
|
出版者 |
Kawasaki Medical Society |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0385-0234 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2434-3404 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12685295 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/KMJ-E44(2)41h |
| 記事種別(英) |
|
|
内容記述 |
Regular Article |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2018en/KMJ-E44(2)41.pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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