| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
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|
タイトル |
Molecular Cloning of the Escherichia coli Glutamine Permease Operon, glnP, Using Mini-F Plasmid |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
NOHNO, Tsutomu
SAITO, Taiichi
Jen-shiang, HONG
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| 著者所属(英) |
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|
en |
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Department of Pharmacology, Kawasaki Medical School |
| 著者所属(英) |
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|
en |
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Department of Pharmacology, Kawasaki Medical School |
| 著者所属(英) |
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|
en |
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Department of Cell Physiology, Boston Biomedical Research Institute |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Glutamine permease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Glutamine binding protein |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
glnP |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
glnH |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Escherichia coli |
| 記事種別(英) |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Original Article |
|
言語 |
en |
| 抄録(英) |
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|
en |
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The genes encoding the Escherichia coli high-affinity transport system for glutamine, glutamine permease operon, were cloned by means of phenotypic complementation using a mini-F cloning vehicle. Plasmid pTN101 contains the structural gene for the periplasmic glutamine binding protein, glnH, and at least one other gene, glnP, also required for the permease activity. It is difficult to reclone the entire operon into high-copy number plasmids pBR322 or pUC18: The resultant plasmids are extremely unstable and are rapidly lost from the host cell. Subcloning of the operon in pBR322 resulted in the loss of Gln+ phenotype, an ability to utilize glutamine as a sole source of carbon. Direct cloning of the permease operon by using pBR322 was unsuccessful; although three types of Gln+ growers were obtained, they do not contain DNA in the glnP locus. |
| 書誌情報 |
en : Kawasaki medical journal
巻 12,
号 4,
p. 163-176,
発行日 1986
|
| URL |
|
|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1986en/12(4)163-176.1986.pdf |
|
識別子タイプ |
URI |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/KMJ-E12(4)163 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0385-0234 |
| ISSN |
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|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2434-3404 |
| 雑誌書誌ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00710169 |
| 雑誌書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
KJ00010222419.pdf (2.4 MB)
