| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-06-26 |
| タイトル |
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|
タイトル |
Pathological analysis of age-related bladder dysfunction |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
NAKATSUKA, Tota
OHIRA, Shin
KAKUMAE, Sho
SHIMIZU, Shinjiro
TONE, Shigenobu
NAGAI, Atsushi
UEHARA, Shinya
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| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Faculty of Science and Engineering, Tokyo Denki University |
| 著者所属(英) |
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言語 |
en |
|
値 |
Director of Hospital, Kawasaki Medical School Hospital |
| 著者所属(英) |
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|
言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Age-related bladder dysfunction |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Pathophysiological analysis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Anti-oxidative stress |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Antioxidant capacity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Oxidative capacity |
| 抄録(英) |
|
|
言語 |
en |
|
値 |
Introduction: Globally, over 50% of adults exhibit lower urinary tract symptoms. While aging is a known risk factor, the pathogenesis of age-related bladder dysfunction remains unclear. Recently, changes in oxidative stress were reported to be associated with the development of age-related bladder dysfunction; therefore, we examined the age-related changes in bladder function in an aging mouse model with a focus on oxidative stress.<br>Materials and Methods: Experimental animals included male C57BL/6J mice aged 20, 40, 60, and 80 weeks. Using 20-week-old mice as the control group, age-related changes in bladder function were verified. Functional changes in the bladder were investigated using a pressure flow study (PFS), and morphohistological changes were analyzed using upright light microscopy and transmission electron microscopy (TEM). Additionally, changes in oxidative stress in the serum and bladder were analyzed using diacron-reactive oxygen metabolite (d-ROM) and biological antioxidant potential (BAP) tests. The localization of oxidative stress in the bladder was analyzed using nitrotyrosine (NTY) and superoxide dismutase 2 (SOD2) immunofluorescence staining.<br>Results: The PFS demonstrated significantly decreased maximum cystometric capacity and maximum detrusor pressure, as well as increased overcontraction of the bladder sphincter muscle and inadequate reduction in detrusor pressure after voiding with age. Masson’s trichrome staining and TEM showed thinning of the transitional epithelium of the bladder, and fibrosis extending from the interstitium to the muscle layer with age. The pixel area ratio of the epithelial layer significantly decreased, while that of the interstitial and muscular layers significantly increased with age in all layers (from epithelial to muscular layers). Oxidative stress analysis revealed a decrease in antioxidant capacity in the serum and bladder, followed by an increase in oxidative capacity, resulting in the overproduction of oxidative stress. Immunofluorescence staining showed that a biphasic decrease in antioxidant capacity (SOD2) preceded an increase in oxidative capacity (NTY) with aging. Analysis of the localization of oxidative stress in the bladder using immunofluorescence staining confirmed highly biphasic staining in the epithelial layer and from the interstitial to the muscular layer, and a biphasic decrease in SOD2 preceded an increase in NTY with aging.<br>Conclusions: A biphasic state of oxidative stress in the bladder epithelial layer and from the interstitial to muscle layer, as well as bladder dysfunction causing a mixture of overactivity and underactivity of the detrusor muscle, were observed with aging. Oxidative stress is preceded by a decrease in antioxidant capacity, followed by an increase in oxidant capacity. These changes suggest that a decrease in antioxidant capacity may serve as a biomarker reflecting the pathology of early-stage age-related bladder dysfunction. |
| 書誌情報 |
en : Kawasaki medical journal
巻 49,
p. 57-67,
発行日 2023
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| 出版者 |
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出版者 |
Kawasaki Medical Society |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
24343404 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.11482/KMJ-E202349057 |
| 記事種別(英) |
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内容記述 |
Regular Article |
|
言語 |
en |
| 関連サイト |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2023en/KMJ-E202349057.pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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