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A Comparison of Analytical Methods for the Generation Time of Cultured Human Myeloma Cell Line, KMM-1
https://kwmed.repo.nii.ac.jp/records/227
https://kwmed.repo.nii.ac.jp/records/2279f9a112f-549f-4c47-bbab-dd30820e6cf2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00010220299.pdf (759.9 kB)
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| Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2017-01-23 | |||||||
| タイトル | ||||||||
| タイトル | A Comparison of Analytical Methods for the Generation Time of Cultured Human Myeloma Cell Line, KMM-1 | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 著者 |
OHTSUKI, TAKEMI
× OHTSUKI, TAKEMI
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| 著者所属(英) | ||||||||
| en | ||||||||
| Division of Hematology, Department of Medicine, Kawasaki Medical School | ||||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | cell growth kinetics | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | human myeloma | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | cell synchronization | |||||||
| 記事種別(英) | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Original Article | |||||||
| 言語 | en | |||||||
| 抄録(英) | ||||||||
| en | ||||||||
| The generation time (GT) of a human myeloma cell line (KMM-1) producing λ-type Bence-Jones protein1) was analyzed by the following methods; calculation from the growth curve, autoradiographic analyses of continuous 3H-thymidine labeling and of a fraction of labeled mitoses, the sister chromatid exchange method, time-lapse cinemicrography, and flow cytometric measurement. The GTs obtained by each method differed considerably. The GT calculated from the cell growth curve was 29.3 hr, while those analyzed by other methods were as follows: 16.6 hr by a fraction of labeled mitoses, 22.9 hr by sister chromatid exchange, and 26.4 hr by time-lapse cinemicrography, and 18 to 20 hr by flow cytometric analysis. The mean GT of the cultured myeloma cells, when averaged from these results, was 22.8 hr. The reasons for differences in the GTs obtained by these different analytical methods were suggested in Discussion. | ||||||||
| 書誌情報 |
en : Kawasaki medical journal 巻 13, 号 4, p. 169-176, 発行日 1987 |
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| URL | ||||||||
| 識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1987en/13(4)169-176.1987.pdf | |||||||
| 識別子タイプ | URI | |||||||
| DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.11482/KMJ-E13(4)169 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 0385-0234 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 2434-3404 | |||||||
| 雑誌書誌ID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00710169 | |||||||
| 雑誌書誌ID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA12029005 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
