| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
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タイトル |
Limitations of Electron Spin Resonance (ESR) Spectroscopy in the Superoxide Dismutase Assay of Blood |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
TOMITA, Masasfumi
TANAKA, Mari
WATANABE, Hiroko
OKUYAMA, Toshiko
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| 著者所属(英) |
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en |
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Department of Legal Medicine, Kawasaki Medicak School |
| 著者所属(英) |
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en |
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Department of Biochemical Research Center, Kawasaki Medical School |
| 著者所属(英) |
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en |
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Department of Biochemical Research Center, Kawasaki Medical School |
| 著者所属(英) |
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en |
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Department of Legal Medicine, Kawasaki Medical School |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Superoxide dismutase |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Electron spin resonance spectroscopy |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Radical scavenger |
| 記事種別(英) |
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内容記述タイプ |
Other |
|
内容記述 |
Original Article |
|
言語 |
en |
| 抄録(英) |
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en |
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We have employed the electron spin resonance spin-trapping method to determine the superoxide dismutase (SOD) activity in plasma and erythrocytes, and investigated various possible obstacles associated with detection of the superoxide radical derived from the hypoxanthinexanthine oxidase system. With the exception of SOD itself, ascorbate, present in plasma at 40-140 μM, diminished the peak for the DMPO-OOH spin adduct most remarkably. Its 50% inhibitory concentration was 115 μM. Cysteine, reduced glutathione (GSH), urate, NADH and 5'-ATP also showed remarkable diminishing effects, although their concentrations in blood were too low to affect the DMPO-OOH spin adduct. Sugars had little or no effect. Hemoglobin and serum albumin showed a significant effect above a concentration of 1.5 and 5 mg/ml, respectively. In addition, ascorbate and albumin each showed a dose-dependent diminishing effect when 1.5 units/ml of SOD was used simultaneously with ascorbate or albumin. Therefore, the presence of ascorbate and albumin was not negligible when a slight amount of SOD activity in plasma was determined directly. The effect of hemoglobin, however, was overcome by dilution because the activity in erythrocytes was sufficiently high. |
| 書誌情報 |
en : Kawasaki medical journal
巻 15,
号 3･4,
p. 133-139,
発行日 1989
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| URL |
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|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1989en/15(3.4)133-139.1989.pdf |
|
識別子タイプ |
URI |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.11482/KMJ-E15(3.4)133 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0385-0234 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2434-3404 |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00710169 |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| 著者版フラグ |
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出版タイプ |
VoR |
KJ00010214959.pdf (672.1 kB)
