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A feasible protocol for identifying macrolide resistant mutations in the 23S rRNA domain V sequence of Mycoplasma pneumoniae
https://kwmed.repo.nii.ac.jp/records/2971
https://kwmed.repo.nii.ac.jp/records/2971fb63fdf3-96f2-4f0b-a169-921494955e87
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
PDF (854.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2022-04-01 | |||||||||
| タイトル | ||||||||||
| タイトル | A feasible protocol for identifying macrolide resistant mutations in the 23S rRNA domain V sequence of Mycoplasma pneumoniae | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
MIYATA, Ippei
× MIYATA, Ippei
× OUCHI, Kazunobu
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| 著者所属(英) | ||||||||||
| 言語 | en | |||||||||
| 値 | Department of Pediatrics, Kawasaki Medical School | |||||||||
| 著者所属(英) | ||||||||||
| 言語 | en | |||||||||
| 値 | Department of Pediatrics, Kawasaki Medical School | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Mycoplasma pneumoniae | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Macrolide resistance | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 23S rRNA | |||||||||
| 抄録(英) | ||||||||||
| 言語 | en | |||||||||
| 値 | Continuing a nationwide surveillance of pediatric Mycoplasma pneumoniae infections since 2008, our department has been analyzing macrolide-resistance conferring mutations in the pathogen’s 23S rRNA gene sequence. There are three target positions, approximately 600 bases apart. We had been reading these positions by amplifying the flanking sequences of these target positions in two different PCR reactions, followed by sequencing of each PCR product, independently. Recent advances have boosted the expected read length of Sanger sequencing using dye-terminators from ~500 bases to ~1 Kb. Owing to a constant demand to process tens of specimens, the authors sought to refine the protocols with an aim to reduce handling time, complexity, and cost. Hereby presented is our refined procedure that enhanced our laboratory’s capacity enormously | |||||||||
| 書誌情報 |
en : Kawasaki medical journal 巻 47, p. 7-11, 発行日 2021 |
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| 出版者 | ||||||||||
| 出版者 | Kawasaki Medical Society | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03850234 | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | EISSN | |||||||||
| 収録物識別子 | 24343404 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA12685295 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA12029005 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.11482/KMJ-E202147007 | |||||||||
| 記事種別(英) | ||||||||||
| 内容記述 | Others | |||||||||
| 言語 | en | |||||||||
| 関連サイト | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2021en/KMJ-E202147007.pdf | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
