| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-03-10 |
| タイトル |
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タイトル |
Preventive effect of indoleamine 2,3-dioxygenase 1 inhibition on lipopolysaccharide-induced prostatitis |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
TAKASAKI, Hiroyasu
OHIRA, Shin
TONE, Shigenobu
KAKUMAE, Sho
NAKATSUKA, Tota
MORINAKA, Hirofumi
HIRATA, Keita
SHIMIZU, Shinjiro
NAGAI, Atsushi
UEHARA, Shinya \n
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| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Faculty of Science and Engineering, Tokyo Denki University |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
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値 |
Director of Hospital, Kawasaki Medical School Hospital |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| キーワード |
|
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Indoleamine 2,3-dioxygenase 1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Prostatitis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Prevention |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Anti-inflammation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
lipopolysaccharide |
| 抄録(英) |
|
|
言語 |
en |
|
値 |
Introduction and Objectives: Bacterial infections are the main cause of acute prostatitis and are treated with appropriate antimicrobial therapy. However, approximately 5% of patients continue to have inflammatory symptoms even after receiving antibacterial therapy, leading to refractory conditions. Bacterial prostatitis requires additional therapy, focusing on inflammatory changes. Indoleamine 2,3-dioxygenase 1 (IDO1) catalysis is the first rate-limiting step of tryptophan metabolism. IDO1 is expressed in the prostate and plays a key role in the immune response. As the first step in investigating the relationship between acute prostatitis and IDO1, we investigated the preventive effect of IDO1 inhibition on lipopolysaccharide (LPS)- induced prostatitis using IDO knockout (Ido1 −/−) mice in this study.
Materials and Methods: The study used Ido1 −/− and wild-type (Ido1 +/+) C57BL/6J male
mice aged 10–15 weeks. LPS Escherichia coli O26 (100μg/PBS, 100μL) was administered transurethrally into the lower urinary tract to create a mouse model of LPS-induced prostatitis. The prostates were removed 1, 3, 5, and 7 days after creating the model mice. Histological, immunohistochemical, and biochemical analyses were used to compare the preventive effect in Ido1 −/− mice compared with that in Ido1+/+ mice.
Results: HE staining showed suppression of ductal destruction following infiltration of inflammatory cells in Ido1 −/− mice compared with Ido1 +/+ mice. The enzyme-linked immunosorbent assay (ELISA) method was used for kynurenine pathway analysis, which showed significantly maintained tryptophan levels and decreased L-kynurenine levels in Ido1 −/− mice compared to Ido1 +/+ mice. The IDO1 assay in Ido1 +/+ mice showed significantly increased levels during all observation periods after creating the model compared with that under normal conditions. Immunofluorescent staining using five types of cytokines and chemokines (IL-2, IL-4, IL-17, CCL2, and CCL3) related to the pathophysiology of acute prostatitis showed decreased expression of these cytokines and chemokines in Ido1 -/- mice compared with Ido1 +/+ mice. Inflammation-related proteome assays showed decreased levels of IL-1β, IL-4, IL-5, IL-6, IL-17, CCL2, CCL3, CXCL1, CXCL11, and tissue inhibitor of matrix metalloproteinases (TIMP)-1 in Ido1 −/− mice compared with Ido1 −/− mice during all observation periods after model creation.
Conclusions: IDO1 is involved in LPS-induced prostatitis through cytokines and chemokines. IDO1 inhibition contributes to the prevention of LPS-induced prostatitis. IDO1 inhibition has the potential to serve as an additional therapy for acute prostatitis. |
| 書誌情報 |
en : Kawasaki medical journal
巻 48,
p. 141-151,
発行日 2022
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| 出版者 |
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出版者 |
Kawasaki Medical Society |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
24343404 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.11482/KMJ-E202248141 |
| 記事種別(英) |
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内容記述 |
Regular Article |
|
言語 |
en |
| 関連サイト |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2022en/KMJ-E202248141.pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
PDF (4.8 MB)
