| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-03-10 |
| タイトル |
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|
タイトル |
Pathophysiological analysis of detrusor overactivity following partial bladder outlet obstruction |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
MORINAKA, Hirofumi
OHIRA, Shin
TONE, Shigenobu
KAKUMAE, Sho
NAKATSUKA, Tota
TAKASAKI, Hiroyasu
HIRATA, Keita
SHIMIZU, Shinjiro
NAGAI, Atsushi
UEHARA, Shinya \n
|
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Faculty of Science and Engineering, Tokyo Denki University |
| 著者所属(英) |
|
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言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| 著者所属(英) |
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|
言語 |
en |
|
値 |
Director of Hospital, Kawasaki Medical School Hospital |
| 著者所属(英) |
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|
言語 |
en |
|
値 |
Department of Urology, Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Detrusor overactivity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Partial bladder outlet obstruction |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bladder dysfunction |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Pathophysiological analysis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Inflammation |
| 抄録(英) |
|
|
言語 |
en |
|
値 |
Introduction: Detrusor overactivity (DO) following partial bladder outlet obstruction (PBOO) is a common urological condition in humans, with 50-70% patients with PBOO complicated with DO. The pathological mechanisms of DO following PBOO are largely unknown, but inflammatory changes may play a key role. We hypothesized that inflammation is important in the earlier pathophysiological phase before overproduction of oxidative stress in DO following PBOO. Therefore, we investigated the relationships among bladder function, ischemia, oxidative stress and inflammation in DO following PBOO in PBOO model mice.
Materials and Methods: C57BL/6J male mice aged 10 to 15 weeks were used in the study. PBOO model mice were created surgically by ligation of the proximal urethra with 5-0 nylon suture under inhalation anesthesia. Sham-operated mice were used as controls. Pathophysiological changes in the bladder at 1, 3 and 5 weeks after creation of the PBOO model mice were compared with those in sham-operated mice using functional, histological, biochemical and immunohistochemical analyses.
Results: Functional analysis using a pressure flow study showed increased maximum detrusor pressure at 1 week and DO from 3 to 5 weeks after creation of the PBOO model. Histological analysis using hematoxylin-eosin and Masson-Trichrome staining showed greater invasion of inflammatory cells and fibrosis in PBOO model mice compared with sham-operated mice at 3 and 5 weeks. Inflammatory cells were mainly present in interstitial tissue, and fibrosis gradually infiltrated from interstitial tissue to the muscular layer. Ischemia analysis showed significantly
increased HIF-1α in PBOO model mice at all time points. Oxidative stress analysis indicated significantly increased levels of ROS from 1 week and 8-OHdG from 3weeks in PBOO model mice. An inflammation-related proteome assay showed high levels of colony stimulating factor (CSF) family proteins at 1 week and IL-2, IL-3, IL-17A, IL-23, MMP-3, MMP-9 and periostin from 3 to 5 weeks in PBOO model mice.
Conclusions: Oxidative stress and inflammatory changes showed contemporaneous increase in pathophysiology of detrusor overactivity following partial bladder outlet obstruction. Especially, CSF family and ROS changes are showed in the early stage, and might be a predict marker in the pathophysiology of DO following PBOO at the early stage. |
| 書誌情報 |
en : Kawasaki medical journal
巻 48,
p. 153-167,
発行日 2022
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| 出版者 |
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|
出版者 |
Kawasaki Medical Society |
| ISSN |
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収録物識別子タイプ |
EISSN |
|
収録物識別子 |
24343404 |
| 書誌レコードID |
|
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.11482/KMJ-E202248153 |
| 記事種別(英) |
|
|
内容記述 |
Regular Article |
|
言語 |
en |
| 関連サイト |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2022en/KMJ-E202248153.pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
PDF (10.1 MB)
