Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
公開日 |
2017-01-23 |
タイトル |
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タイトル |
Detection and Differentiation of Malaria Parasites in DNA Extracted from Blood Samples by the Polymerase Chain Reaction (PCR) |
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言語 |
en |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
著者 |
HARANO, Keiko
Than, Aung Myint
SUETSUGU, Yoshimasa
KAWABATA, Masato
HARANO, Teruo
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著者所属(英) |
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en |
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Department of Clinical Nutrition, Faculty of Medical Professions, Kawasaki University of Medical Welfare |
著者所属(英) |
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en |
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Department of Biochemistry, Kawasaki Medical School |
著者所属(英) |
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en |
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Department of Biochemistry, Kawasaki Medical School |
著者所属(英) |
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en |
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International Center for Medical Research, Kobe University, Graduate School of Medicine, School of Medicine |
著者所属(英) |
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en |
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Department of Biochemistry, Kawasaki Medical School |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Plasmodium falciparum |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Plasmodium vivax |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
polymerase chain reaction (PCR) |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Hb J-Tongariki |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Hb I-Toulouse |
抄録(英) |
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en |
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The purpose of this work was to construct a screening method to detect the small subunit rRNA of malaria parasites, especially Plasmodium falciparum and P. vivax, in blood samples. The PCR method, using the nested multiplex primer set that has been effective for the detection and differentiation of both P. falciparum and P. vivax, was applied to DNA extracted from patients of the Solomon Islands and Papua New Guinea with abnormal hemoglobin, Hb J-Tongariki or Hb I-Toulouse. Since it was, however, not enough to certify the respective Plasmodia in patients infected by multiple species, confirmation by the PCR method using a specific primer set required to detect the respective Plasmodia finally might be necessary. |
書誌情報 |
en : Kawasaki medical journal
巻 27,
号 3・4,
p. 83-89,
発行日 2001
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URL |
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識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2001en/27(3.4)83-89.2001.pdf |
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識別子タイプ |
URI |
DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.11482/KMJ27(3.4)83-89.2001.pdf |
ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0385-0234 |
ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2434-3404 |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00710169 |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12029005 |
著者版フラグ |
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出版タイプ |
VoR |