| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
|
|
タイトル |
Development of two types of mite-allergen induced murine models of chronic asthma with different severity |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
IKEDA, Masaki
KATOH, Shigeki
SHIMIZU, Hiroki
OKA, Mikio
|
| 著者所属(英) |
|
|
|
en |
|
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Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Respiratory Medicine, Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Severity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mite-Allergen |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mouse Model |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Chronic Asthma |
| 記事種別(英) |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Regular Article |
|
言語 |
en |
| 抄録(英) |
|
|
|
en |
|
|
Asthma is an allergic disease characterized by chronic airway inflammation, hyper-responsiveness (AHR), and reversible obstruction. The main inflammatory changes are induced by infiltration of eosinophils into the airway. Few animal models resemble the spontaneous history of asthma due to variations in the selection of the mouse strain, appropriate antigen, and exposure methods. Here, we prepared two different mouse models in which the mechanism was close to that of human asthma. We transnasally administered mite Dermatophagoides farinae (Df) allergen to BALB/c mice 10 times (Df-2) or 25 times (Df-5). After comparison with mice administered phosphate-buffered saline, the AHR and immediate asthmatic response were evaluated, in addition to the number of eosinophils in the bronchoalveolar lavage fluid (BALF). Df-specific IgE and IgG1 levels in the serum, and Th2 cytokines (interleukin [IL]-5, IL-13) in the BALF were measured by enzymelinked immunosorbent assay. Immediate asthmatic response and AHR were enhanced in mite allergen-treated mice (Df-2 and Df-5) compared to PBS-treated mice. The number of eosinophils and IL-13 levels in the BALF, and specific IgE in the serum were greater in Df-5 than in Df-2 mice. We established two different murine chronic asthma models, in which the severity depended on the number of exposures to Df. Greater intranasal exposure to a Df allergen resulted in more severe asthma in a BALB/c mouse model. |
| 書誌情報 |
en : Kawasaki medical journal
巻 42,
号 1,
p. 1-7,
発行日 2016
|
| URL |
|
|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2016en/KMJ-E42(1)1.pdf |
|
識別子タイプ |
URI |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/KMJ-E42(1)1 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0385-0234 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2434-3404 |
| 雑誌書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12685295 |
| 雑誌書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
KJ00010236099.pdf (1.1 MB)
