WEKO3
アイテム
{"_buckets": {"deposit": "ac57f374-3509-4adf-a115-b44cdde75ac4"}, "_deposit": {"created_by": 3, "id": "122", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "122"}, "status": "published"}, "_oai": {"id": "oai:kwmed.repo.nii.ac.jp:00000122", "sets": ["338"]}, "author_link": ["127311", "127310"], "item_3_biblio_info_12": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1984", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "180", "bibliographicPageStart": "169", "bibliographicVolumeNumber": "10", "bibliographic_titles": [{}, {"bibliographic_title": "Kawasaki medical journal", "bibliographic_titleLang": "en"}]}]}, "item_3_description_15": {"attribute_name": "表示順", "attribute_value_mlt": [{"subitem_description": "3", "subitem_description_type": "Other"}]}, "item_3_description_16": {"attribute_name": "アクセション番号", "attribute_value_mlt": [{"subitem_description": "KJ00010225139", "subitem_description_type": "Other"}]}, "item_3_description_9": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_description": "Original Article", "subitem_description_type": "Other"}]}, "item_3_identifier_14": {"attribute_name": "URL", "attribute_value_mlt": [{"subitem_identifier_type": "URI", "subitem_identifier_uri": "http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1984en/10(3)169-180.1984.pdf | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1984en/10(3)169-180.1984.pdf"}]}, "item_3_relation_20": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.11482/KMJ-E10(3)169 ", "subitem_relation_type_select": "DOI"}}]}, "item_3_source_id_1": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00710169", "subitem_source_identifier_type": "NCID"}, {"subitem_source_identifier": "AA12029005", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_19": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0385-0234", "subitem_source_identifier_type": "ISSN"}, {"subitem_source_identifier": "2434-3404", "subitem_source_identifier_type": "ISSN"}]}, "item_3_text_3": {"attribute_name": "論文名よみ", "attribute_value_mlt": [{"subitem_text_value": "Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115"}]}, "item_3_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Pharmacology, Kawasaki Medical School"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Pharmacology, Kawasaki Medical School"}]}, "item_3_textarea_11": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_textarea_language": "en", "subitem_textarea_value": "The effects of molybdate on the binding characteristics of androgen receptor from the androgen-dependent mouse tumor (Shionogi carcinoma 115) with 17/β-hydroxy-5α-androstan-3-one were studied by the charcoal adsorption method. The dissociation constant (Kd) was (4.65±0.54) ×1O-11 M in the presence of 10-12 mM sodium molybdate, which was one order of magnitude lower than the value without molybdate ((4.54±0.72)× 10-10 M). Since the half life of the binding activity of unbound receptor was extended from 10 h to 75 h by adding molybdate, the effects of chaotropic reagents on binding were studied in the presence of molybdate. KC1 (0.3 -1.0 M) remarkably increased Kd without affecting the maximum binding capacity or the dissociation rate. KSCN (\u003e0.1 M) had no effect on Kd but increased the dissociation rate, and while at concentrations at or above 0.5 M it completely inhibited the androgen binding to the cytosol receptor. Urea (0.5-2.0 M) increased Kd proportionally to the increase in the dissociation rate. Kd was also increased by 0.2-0.5 M guanidine, but the maximum binding capacity was reduced. "}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "NOHNO, Tsutomu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127310", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SAITO, Taiichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "127311", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-01-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00010225139.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "KJ00010225139.pdf", "url": "https://kwmed.repo.nii.ac.jp/record/122/files/KJ00010225139.pdf"}, "version_id": "6d71b633-1d7a-43a6-8f37-9c7afde10fd8"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Androgen receptor", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sodium molybdate", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Steroid binding", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Shionogi carcinoma 115", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115", "subitem_title_language": "en"}]}, "item_type_id": "3", "owner": "3", "path": ["338"], "permalink_uri": "https://kwmed.repo.nii.ac.jp/records/122", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-01-23"}, "publish_date": "2017-01-23", "publish_status": "0", "recid": "122", "relation": {}, "relation_version_is_last": true, "title": ["Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115"], "weko_shared_id": -1}
Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115
https://kwmed.repo.nii.ac.jp/records/122
https://kwmed.repo.nii.ac.jp/records/122169b4ae2-4bdb-4176-bd8b-0e0faa32f1d0
名前 / ファイル | ライセンス | アクション |
---|---|---|
KJ00010225139.pdf (1.1 MB)
|
|
Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-01-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
NOHNO, Tsutomu
× NOHNO, Tsutomu× SAITO, Taiichi |
|||||
著者所属(英) | ||||||
en | ||||||
Department of Pharmacology, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pharmacology, Kawasaki Medical School | ||||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Androgen receptor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sodium molybdate | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Steroid binding | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Shionogi carcinoma 115 | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Original Article | |||||
抄録(英) | ||||||
en | ||||||
The effects of molybdate on the binding characteristics of androgen receptor from the androgen-dependent mouse tumor (Shionogi carcinoma 115) with 17/β-hydroxy-5α-androstan-3-one were studied by the charcoal adsorption method. The dissociation constant (Kd) was (4.65±0.54) ×1O-11 M in the presence of 10-12 mM sodium molybdate, which was one order of magnitude lower than the value without molybdate ((4.54±0.72)× 10-10 M). Since the half life of the binding activity of unbound receptor was extended from 10 h to 75 h by adding molybdate, the effects of chaotropic reagents on binding were studied in the presence of molybdate. KC1 (0.3 -1.0 M) remarkably increased Kd without affecting the maximum binding capacity or the dissociation rate. KSCN (>0.1 M) had no effect on Kd but increased the dissociation rate, and while at concentrations at or above 0.5 M it completely inhibited the androgen binding to the cytosol receptor. Urea (0.5-2.0 M) increased Kd proportionally to the increase in the dissociation rate. Kd was also increased by 0.2-0.5 M guanidine, but the maximum binding capacity was reduced. | ||||||
書誌情報 |
en : Kawasaki medical journal 巻 10, 号 3, p. 169-180, 発行日 1984 |
|||||
URL | ||||||
識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1984en/10(3)169-180.1984.pdf | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1984en/10(3)169-180.1984.pdf | |||||
識別子タイプ | URI | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11482/KMJ-E10(3)169 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-0234 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2434-3404 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00710169 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12029005 |