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Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115
https://kwmed.repo.nii.ac.jp/records/122
https://kwmed.repo.nii.ac.jp/records/122169b4ae2-4bdb-4176-bd8b-0e0faa32f1d0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00010225139.pdf (1.1 MB)
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| Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2017-01-23 | |||||||||
| タイトル | ||||||||||
| タイトル | Effect of Sodium Molybdate on Androgen Binding to Its Receptor from Shionogi Carcinoma 115 | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
NOHNO, Tsutomu
× NOHNO, Tsutomu
× SAITO, Taiichi
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| 著者所属(英) | ||||||||||
| en | ||||||||||
| Department of Pharmacology, Kawasaki Medical School | ||||||||||
| 著者所属(英) | ||||||||||
| en | ||||||||||
| Department of Pharmacology, Kawasaki Medical School | ||||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Androgen receptor | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Sodium molybdate | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Steroid binding | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Shionogi carcinoma 115 | |||||||||
| 記事種別(英) | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Original Article | |||||||||
| 言語 | en | |||||||||
| 抄録(英) | ||||||||||
| en | ||||||||||
| The effects of molybdate on the binding characteristics of androgen receptor from the androgen-dependent mouse tumor (Shionogi carcinoma 115) with 17/β-hydroxy-5α-androstan-3-one were studied by the charcoal adsorption method. The dissociation constant (Kd) was (4.65±0.54) ×1O-11 M in the presence of 10-12 mM sodium molybdate, which was one order of magnitude lower than the value without molybdate ((4.54±0.72)× 10-10 M). Since the half life of the binding activity of unbound receptor was extended from 10 h to 75 h by adding molybdate, the effects of chaotropic reagents on binding were studied in the presence of molybdate. KC1 (0.3 -1.0 M) remarkably increased Kd without affecting the maximum binding capacity or the dissociation rate. KSCN (>0.1 M) had no effect on Kd but increased the dissociation rate, and while at concentrations at or above 0.5 M it completely inhibited the androgen binding to the cytosol receptor. Urea (0.5-2.0 M) increased Kd proportionally to the increase in the dissociation rate. Kd was also increased by 0.2-0.5 M guanidine, but the maximum binding capacity was reduced. | ||||||||||
| 書誌情報 |
en : Kawasaki medical journal 巻 10, 号 3, p. 169-180, 発行日 1984 |
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| URL | ||||||||||
| 識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/1984en/10(3)169-180.1984.pdf | |||||||||
| 識別子タイプ | URI | |||||||||
| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.11482/KMJ-E10(3)169 | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 0385-0234 | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | EISSN | |||||||||
| 収録物識別子 | 2434-3404 | |||||||||
| 雑誌書誌ID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA00710169 | |||||||||
| 雑誌書誌ID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA12029005 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
