WEKO3
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S1PR1 is strongly expressed in angiosarcoma, a malignant tumor of endothelial cell origin that has a high propensity for metastasis and poor prognosis; however, the pathological significance of S1PR1 expression is not clear. In this study, we investigated the effect of S1PR1 modulation on cell migration, and examined its potential role as a therapeutic target against metastatic dissemination of angiosarcoma. S1PR1 expression in the human angiosarcoma cell line MO-LAS was assessed by immunocytochemical examination and Western blotting. Effects of S1PR1- specific small interfering RNA (siRNA) and that of FTY720-P (a functional S1PR1-antagonist) on MO-LAS cell chemotactic migration towards sphingosine-1-phosphate (S1P) or 10% fetal bovine serum (FBS) were assessed by Transwell migration assay; wound healing assays for random cell migration were performed using a live cell analyzer. Immunostaining revealed high expression of S1PR1 on the MO-LAS cell membrane. 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Sphingosine-1-phosphate receptor 1 expression in angiosarcoma : Possible role in metastasis and a potential therapeutic target
https://kwmed.repo.nii.ac.jp/records/1830
https://kwmed.repo.nii.ac.jp/records/1830b60e1be7-b652-4b89-b478-d6eb13ee9193
名前 / ファイル | ライセンス | アクション |
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PDF (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-03-09 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Sphingosine-1-phosphate receptor 1 expression in angiosarcoma : Possible role in metastasis and a potential therapeutic target | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
OKA, Daigo
× OKA, Daigo× KORESAWA, Risa× FUJIWARA, Hideyo× NISHIMURA, Hirotake× IREI, Isao× AKIYAMA, Takashi× HAMAZAKI, Shuji× FUJIMOTO, Wataru× MASUZAWA, Mikio× SADAHIRA, Yoshito |
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著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Dermatology, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Molecular Diagnostics, Kitasato University School of Allied Health Sciences | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Pathology 1, Kawasaki Medical School | ||||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Angiosarcoma | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sphingosine-1-phosphate receptor 1 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | FTY720-phosphate | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Migration | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Metastasis | |||||
抄録(英) | ||||||
en | ||||||
Sphingosine-1-phosphate (S1P) is a potent lipid mediator that has been implicated in the migration of lymphocytes and endothelial cells through S1P receptors. S1PR1 is strongly expressed in angiosarcoma, a malignant tumor of endothelial cell origin that has a high propensity for metastasis and poor prognosis; however, the pathological significance of S1PR1 expression is not clear. In this study, we investigated the effect of S1PR1 modulation on cell migration, and examined its potential role as a therapeutic target against metastatic dissemination of angiosarcoma. S1PR1 expression in the human angiosarcoma cell line MO-LAS was assessed by immunocytochemical examination and Western blotting. Effects of S1PR1- specific small interfering RNA (siRNA) and that of FTY720-P (a functional S1PR1-antagonist) on MO-LAS cell chemotactic migration towards sphingosine-1-phosphate (S1P) or 10% fetal bovine serum (FBS) were assessed by Transwell migration assay; wound healing assays for random cell migration were performed using a live cell analyzer. Immunostaining revealed high expression of S1PR1 on the MO-LAS cell membrane. Transwell and wound-healing assays showed that S1P enhanced chemotactic and random migration of MO-LAS cells, respectively. Inhibition of S1PR1 expression with siRNA significantly attenuated chemotaxis of cells towards S1P and 10% FBS. Further, FTY720-P strongly induced the internalization and degradation of S1PR1 even in the presence of serum containing S1P. It attenuated chemotactic cell migration of MO-LAS towards both S1P and serum, as well as the random motility of cells at nanomolar concentrations. These results suggest that the S1P/S1PR1 axis may be a potential therapeutic target for inhibition of angiosarcoma metastasis by controlling its cell motility. | ||||||
書誌情報 |
en : Kawasaki Medical Journal 巻 42, 号 2, p. 31-45, 発行日 2016 |
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出版者 | ||||||
出版者 | Kawasaki Medical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-0234 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2434-3404 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12685295 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12029005 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11482/KMJ-E42(2)31 | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Regular Article | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |