| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-03-09 |
| タイトル |
|
|
タイトル |
Sphingosine-1-phosphate receptor 1 expression in angiosarcoma : Possible role in metastasis and a potential therapeutic target |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
OKA, Daigo
KORESAWA, Risa
FUJIWARA, Hideyo
NISHIMURA, Hirotake
IREI, Isao
AKIYAMA, Takashi
HAMAZAKI, Shuji
FUJIMOTO, Wataru
MASUZAWA, Mikio
SADAHIRA, Yoshito
|
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Dermatology, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Molecular Diagnostics, Kitasato University School of Allied Health Sciences |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Pathology 1, Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Angiosarcoma |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Sphingosine-1-phosphate receptor 1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
FTY720-phosphate |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Migration |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Metastasis |
| 抄録(英) |
|
|
言語 |
en |
|
値 |
Sphingosine-1-phosphate (S1P) is a potent lipid mediator that has been implicated in the migration of lymphocytes and endothelial cells through S1P receptors. S1PR1 is strongly expressed in angiosarcoma, a malignant tumor of endothelial cell origin that has a high propensity for metastasis and poor prognosis; however, the pathological significance of S1PR1 expression is not clear. In this study, we investigated the effect of S1PR1 modulation on cell migration, and examined its potential role as a therapeutic target against metastatic dissemination of angiosarcoma. S1PR1 expression in the human angiosarcoma cell line MO-LAS was assessed by immunocytochemical examination and Western blotting. Effects of S1PR1- specific small interfering RNA (siRNA) and that of FTY720-P (a functional S1PR1-antagonist) on MO-LAS cell chemotactic migration towards sphingosine-1-phosphate (S1P) or 10% fetal bovine serum (FBS) were assessed by Transwell migration assay; wound healing assays for random cell migration were performed using a live cell analyzer. Immunostaining revealed high expression of S1PR1 on the MO-LAS cell membrane. Transwell and wound-healing assays showed that S1P enhanced chemotactic and random migration of MO-LAS cells, respectively. Inhibition of S1PR1 expression with siRNA significantly attenuated chemotaxis of cells towards S1P and 10% FBS. Further, FTY720-P strongly induced the internalization and degradation of S1PR1 even in the presence of serum containing S1P. It attenuated chemotactic cell migration of MO-LAS towards both S1P and serum, as well as the random motility of cells at nanomolar concentrations. These results suggest that the S1P/S1PR1 axis may be a potential therapeutic target for inhibition of angiosarcoma metastasis by controlling its cell motility. |
| 書誌情報 |
en : Kawasaki Medical Journal
巻 42,
号 2,
p. 31-45,
発行日 2016
|
| 出版者 |
|
|
出版者 |
Kawasaki Medical Society |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0385-0234 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2434-3404 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12685295 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/KMJ-E42(2)31 |
| 記事種別(英) |
|
|
内容記述 |
Regular Article |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
PDF (1.3 MB)
