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The efficient detection of membrane protein with immunoblotting: lessons from cold-temperature denaturation
https://kwmed.repo.nii.ac.jp/records/2972
https://kwmed.repo.nii.ac.jp/records/2972f116af19-984d-4d11-834b-e31c49fd82b6
名前 / ファイル | ライセンス | アクション |
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PDF (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-04-01 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The efficient detection of membrane protein with immunoblotting: lessons from cold-temperature denaturation | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
MIYANO, Kei
× MIYANO, Kei× OKAMOTO, Shuichiro× KAJIKAWA, Mizuho× KAWAI, Chikage× KANAGAWA, Tomoka× TOMINAGA, Sayuri× YAMAUCHI, Akira× KURIBAYASHI, Futoshi |
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著者所属(英) | ||||||
en | ||||||
Department of Biochemistry, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Biochemistry, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Laboratory of Microbiology, Showa Pharmaceutical University | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Biochemistry, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Second Year Medical Student in fiscal year of 2020, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Second Year Medical Student in fiscal year of 2020, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Biochemistry, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Biochemistry, Kawasaki Medical School | ||||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Membrane protein | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Endoglycosidase, Immunoblotting | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | SDS-PAGE, VEGFR-2 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | NADPH oxidase 4 | |||||
抄録(英) | ||||||
en | ||||||
Transmembrane proteins play essential roles in cell signaling, transport of membrane-impermeable molecules, cell-cell communication, and cell adhesion. Our recent work demonstrated that reactive oxygen species-generating NADPH oxidase 4 (Nox4), a protein with multiple transmembrane domains, is involved in cell migration by stabilizing vascular endothelial growth factor receptor 2 (VEGFR-2), a single-span transmembrane protein. During this study and with further verification, we developed a simple method to prepare protein samples without aggregating these membrane proteins for SDS-PAGE, immunoblotting, and deglycosylation assay. We found that heating was unnecessary for protein denaturation for SDS-PAGE and deglycosylation assay. Also, the detectable amounts of VEGFR-2 and Nox4 were increased in the sample treated at 4℃ compared with the sample treated at 98℃ . Moreover, the N-glycan of VEGFR-2 was digested by glycosidase at reaction temperature 4℃ . | ||||||
書誌情報 |
en : Kawasaki medical journal 巻 47, p. 13-19, 発行日 2021 |
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出版者 | ||||||
出版者 | Kawasaki Medical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03850234 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 24343404 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12685295 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12029005 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11482/KMJ-E202147013 | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Regular Article | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2021en/KMJ-E202147013.pdf | |||||
関連名称 | https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2021en/KMJ-E202147013.pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |