| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-04-01 |
| タイトル |
|
|
タイトル |
The efficient detection of membrane protein with immunoblotting: lessons from cold-temperature denaturation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
MIYANO, Kei
OKAMOTO, Shuichiro
KAJIKAWA, Mizuho
KAWAI, Chikage
KANAGAWA, Tomoka
TOMINAGA, Sayuri
YAMAUCHI, Akira
KURIBAYASHI, Futoshi
|
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Biochemistry, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Biochemistry, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Laboratory of Microbiology, Showa Pharmaceutical University |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Biochemistry, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Second Year Medical Student in fiscal year of 2020, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Second Year Medical Student in fiscal year of 2020, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Biochemistry, Kawasaki Medical School |
| 著者所属(英) |
|
|
言語 |
en |
|
値 |
Department of Biochemistry, Kawasaki Medical School |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Membrane protein |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Endoglycosidase, Immunoblotting |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
SDS-PAGE, VEGFR-2 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NADPH oxidase 4 |
| 抄録(英) |
|
|
言語 |
en |
|
値 |
Transmembrane proteins play essential roles in cell signaling, transport of membrane-impermeable molecules, cell-cell communication, and cell adhesion. Our recent work demonstrated that reactive oxygen species-generating NADPH oxidase 4 (Nox4), a protein with multiple transmembrane domains, is involved in cell migration by stabilizing vascular endothelial growth factor receptor 2 (VEGFR-2), a single-span transmembrane protein. During this study and with further verification, we developed a simple method to prepare protein samples without aggregating these membrane proteins for SDS-PAGE, immunoblotting, and deglycosylation assay. We found that heating was unnecessary for protein denaturation for SDS-PAGE and deglycosylation assay. Also, the detectable amounts of VEGFR-2 and Nox4 were increased in the sample treated at 4℃ compared with the sample treated at 98℃ . Moreover, the N-glycan of VEGFR-2 was digested by glycosidase at reaction temperature 4℃ . |
| 書誌情報 |
en : Kawasaki medical journal
巻 47,
p. 13-19,
発行日 2021
|
| 出版者 |
|
|
出版者 |
Kawasaki Medical Society |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03850234 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
24343404 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12685295 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11482/KMJ-E202147013 |
| 記事種別(英) |
|
|
内容記述 |
Regular Article |
|
言語 |
en |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2021en/KMJ-E202147013.pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
PDF (1.2 MB)
