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Simple and rapid detection method for qepA1 by loop-mediated isothermal amplification
https://kwmed.repo.nii.ac.jp/records/588
https://kwmed.repo.nii.ac.jp/records/58832757db9-4a36-433e-9436-dae43b0b9a74
名前 / ファイル | ライセンス | アクション |
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KJ00010242639.pdf (690.0 kB)
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Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-01-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Simple and rapid detection method for qepA1 by loop-mediated isothermal amplification | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
YAMANE, Kunikazu
× YAMANE, Kunikazu× HORINO, Atsuko× SUZUKI, Satowa× SHIBAYAMA, Keigo× FUSHIMI, Shigeko× KATSUYAMA, Hironobu |
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著者所属(英) | ||||||
en | ||||||
Department of Public Health, Kawasaki Medical School・Department of Bacteriology II, National Institute of Infectious Diseases・Department of Infectious Diseases, Yonago Medical Center, National Hospital Organization | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Bacteriology II, National Institute of Infectious Diseases | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Bacteriology II, National Institute of Infectious Diseases | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Bacteriology II, National Institute of Infectious Diseases | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Public Health, Kawasaki Medical School | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Public Health, Kawasaki Medical School | ||||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | QepA1 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Plasmid-mediated Quinolone Resistance | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | LAMP | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Regular Article | |||||
言語 | en | |||||
抄録(英) | ||||||
en | ||||||
Although fluoroquinolone (FQ) has been used for the treatment of various bacterial infectious diseases, its continued use has been problematic given the appearance of FQ-resistant bacteria. However, the recent discovery of four plasmid-mediated quinolone resistance (PMQR) genes comprising qnr, aac(6')Ib-cr, qepA and OqxAB since 1998 has provided insights in the area of FQ-resistance. For practical detection of qepA in microbiology laboratory, a specific, simple, rapid and cost-effective isothermal amplification method designated as LAMP is the good candidate to use. In this study, the development of a new detection method using LAMP to identify qepA1, one variant of the qepA gene, was tried. As the results, the LAMP method using a qepA1-specific LAMP primer set comprising five primerscould detect all four qepA1-positive strains in addition to 17 qepA1-negative strains. The LAMP method is clearly much more advantageous for use in clinical laboratories. Furthermore, the time and accuracy benefits allow for the selection of antibiotics in a clinical setting. | ||||||
書誌情報 |
en : Kawasaki medical journal 巻 42, 号 1, p. 25-29, 発行日 2016 |
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URL | ||||||
識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2016en/KMJ-E42(1)25.pdf | |||||
識別子タイプ | URI | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11482/KMJ-E42(1)25 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0385-0234 | |||||
ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2434-3404 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12685295 | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12029005 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |