| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-01-23 |
| タイトル |
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|
タイトル |
Simple and rapid detection method for qepA1 by loop-mediated isothermal amplification |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
YAMANE, Kunikazu
HORINO, Atsuko
SUZUKI, Satowa
SHIBAYAMA, Keigo
FUSHIMI, Shigeko
KATSUYAMA, Hironobu
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| 著者所属(英) |
|
|
|
en |
|
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Department of Public Health, Kawasaki Medical School・Department of Bacteriology II, National Institute of Infectious Diseases・Department of Infectious Diseases, Yonago Medical Center, National Hospital Organization |
| 著者所属(英) |
|
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en |
|
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Department of Bacteriology II, National Institute of Infectious Diseases |
| 著者所属(英) |
|
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|
en |
|
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Department of Bacteriology II, National Institute of Infectious Diseases |
| 著者所属(英) |
|
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en |
|
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Department of Bacteriology II, National Institute of Infectious Diseases |
| 著者所属(英) |
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en |
|
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Department of Public Health, Kawasaki Medical School |
| 著者所属(英) |
|
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en |
|
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Department of Public Health, Kawasaki Medical School |
| キーワード |
|
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
QepA1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Plasmid-mediated Quinolone Resistance |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
LAMP |
| 記事種別(英) |
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内容記述タイプ |
Other |
|
内容記述 |
Regular Article |
|
言語 |
en |
| 抄録(英) |
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|
en |
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Although fluoroquinolone (FQ) has been used for the treatment of various bacterial infectious diseases, its continued use has been problematic given the appearance of FQ-resistant bacteria. However, the recent discovery of four plasmid-mediated quinolone resistance (PMQR) genes comprising qnr, aac(6')Ib-cr, qepA and OqxAB since 1998 has provided insights in the area of FQ-resistance. For practical detection of qepA in microbiology laboratory, a specific, simple, rapid and cost-effective isothermal amplification method designated as LAMP is the good candidate to use. In this study, the development of a new detection method using LAMP to identify qepA1, one variant of the qepA gene, was tried. As the results, the LAMP method using a qepA1-specific LAMP primer set comprising five primerscould detect all four qepA1-positive strains in addition to 17 qepA1-negative strains. The LAMP method is clearly much more advantageous for use in clinical laboratories. Furthermore, the time and accuracy benefits allow for the selection of antibiotics in a clinical setting. |
| 書誌情報 |
en : Kawasaki medical journal
巻 42,
号 1,
p. 25-29,
発行日 2016
|
| URL |
|
|
識別子 |
http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2016en/KMJ-E42(1)25.pdf |
|
識別子タイプ |
URI |
| DOI |
|
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.11482/KMJ-E42(1)25 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0385-0234 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2434-3404 |
| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12685295 |
| 雑誌書誌ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12029005 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
KJ00010242639.pdf (690.0 kB)
