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Genistein affects osteoblastic MC3T3-E1 cells both through estrogen receptor and BMP-Smad signaling pathways
https://kwmed.repo.nii.ac.jp/records/1631
https://kwmed.repo.nii.ac.jp/records/16319114b49a-28ce-470c-b04f-2ba14aa714aa
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00008396999.pdf (614.9 kB)
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| Item type | [ELS]学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2017-01-23 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Genistein affects osteoblastic MC3T3-E1 cells both through estrogen receptor and BMP-Smad signaling pathways | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
Hinenoya, Hajime
× Hinenoya, Hajime
× Katsuyama, Hironobu
× Nohno, Tsutomu
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| 著者所属(英) | ||||||||||||
| en | ||||||||||||
| Department of Public Health, Kawasaki Medical School | ||||||||||||
| 著者所属(英) | ||||||||||||
| en | ||||||||||||
| Department of Public Health, Kawasaki Medical School | ||||||||||||
| 著者所属(英) | ||||||||||||
| en | ||||||||||||
| Department of Molecular and Developmental Biology, Kawasaki Medical School | ||||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Genistein | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Osteoprotegerin | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | BMP-Smad signaling | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | MC3T3-E1 cells | |||||||||||
| 記事種別(日) | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | 原著 | |||||||||||
| 言語 | ja | |||||||||||
| 抄録(英) | ||||||||||||
| en | ||||||||||||
| Many epidemiological studies show that genistein intake is effective for maintaining bone mineral density (BMD). Because the reason for the efficacy of genistein as a bone protective agent in vivo remains unclear, we investigated the mechanisms underlying the effects of genistein on BMD in relation to BMP-Smad signaling systems. When osteoblastic MC3T3-E1 cells were exposed to 1 μM genistein, they increased in number. Combined administrations of 1 μM genistein and 1 μM of ICI 182,780 inhibited the increase in cell numbers. Alkaline phosphatase (ALP) and Alizarin red staining showed high activities, indicating that genistein might promote estrogenic differentiation of MC3T3-E1 cells. Moreover, ELISA determined that production of osteoprotegerin (OPG), which is expressed by osteoblasts, was higher when 1 μM genistein was added to the medium than in controls. In contrast, when 10 ng/mL of noggin was administered in the medium, OPG production was inhibited. In order to clarify the underlying mechanism, we investigated the BMP-Smad signaling pathway. When genistein was added to the medium, it induced gene expression of BMP-4. Immunofluorescence staining showed that genistein induced phosphorylation of Smad 1/5, a downstream molecule of BMP. When noggin, which binds to BMP and blocks BMP signaling, was added to the medium, phosphorylation of Smad 1/5 was reduced. These results indicate that genistein may regulate bone metabolism through the BMP-Smad signaling pathway as well as through the estrogen receptor pathway. | ||||||||||||
| 書誌情報 |
ja : 川崎医学会誌 en : Kawasaki medical journal 巻 39, 号 1, p. 21-31, 発行日 2013 |
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| URL | ||||||||||||
| 識別子 | http://igakkai.kms-igakkai.com/wp/wp-content/uploads/2013/KMJ31(1)21-31.2013.pdf | |||||||||||
| 識別子タイプ | URI | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.11482/2013/KMJ31(1)21-31.2013.pdf | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 0386-5924 | |||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||
| 収録物識別子 | 2758-089X | |||||||||||
| 雑誌書誌ID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AN00045593 | |||||||||||
| 雑誌書誌ID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AN12940574 | |||||||||||
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| 出版タイプ | VoR | |||||||||||
